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Mannose impairs tumour growth and enhances chemotherapy

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Title: Mannose impairs tumour growth and enhances chemotherapy
Authors: Sierra Gonzalez, P
O’Prey, J
Cardaci, S
Barthet, V
Sakamaki, J-I
Beaumatin, F
Roseweir, A
Gay, D
Mackay, G
Malviya, G
Kania, E
Ritchie, S
Baudot, A
Zunino, B
Mrowinska, A
Nixon, C
Ennis, D
Hoyle, A
Millan, D
McNeish, I
Sansom, O
Edwards, J
Ryan, K
Item Type: Journal Article
Abstract: It is now well established that tumours undergo changes in cellular metabolism1. As this can reveal tumour cell vulnerabilities and because many tumours exhibit enhanced glucose uptake2, we have been interested in how tumour cells respond to different forms of sugar. Here we report that the monosaccharide mannose causes growth retardation in several tumour types in vitro, and enhances cell death in response to major forms of chemotherapy. We then show that these effects also occur in vivo in mice following the oral administration of mannose, without significantly affecting the weight and health of the animals. Mechanistically, mannose is taken up by the same transporter(s) as glucose3 but accumulates as mannose-6-phosphate in cells, and this impairs the further metabolism of glucose in glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway and glycan synthesis. As a result, the administration of mannose in combination with conventional chemotherapy affects levels of anti-apoptotic proteins of the Bcl-2 family, leading to sensitization to cell death. Finally we show that susceptibility to mannose is dependent on the levels of phosphomannose isomerase (PMI). Cells with low levels of PMI are sensitive to mannose, whereas cells with high levels are resistant, but can be made sensitive by RNA-interference-mediated depletion of the enzyme. In addition, we use tissue microarrays to show that PMI levels also vary greatly between different patients and different tumour types, indicating that PMI levels could be used as a biomarker to direct the successful administration of mannose. We consider that the administration of mannose could be a simple, safe and selective therapy in the treatment of cancer, and could be applicable to multiple tumour types.
Issue Date: 21-Nov-2018
Date of Acceptance: 5-Oct-2018
URI: http://hdl.handle.net/10044/1/65247
DOI: https://dx.doi.org/10.1038/s41586-018-0729-3
ISSN: 0028-0836
Publisher: Nature Publishing Group
Start Page: 719
End Page: 723
Journal / Book Title: Nature
Volume: 563
Copyright Statement: © 2018 Springer Nature Limited. All rights reserved.
Sponsor/Funder: Cancer Research UK
Funder's Grant Number: RG71079
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
CELL-SURVIVAL
METABOLISM
AUTOPHAGY
RESISTANCE
APOPTOSIS
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Appears in Collections:Division of Surgery
Division of Cancer
Faculty of Medicine



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