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Defective presentation to class I restricted CTL in vaccinia infected cells is overcome by enhanced degradation of antigen

Title: Defective presentation to class I restricted CTL in vaccinia infected cells is overcome by enhanced degradation of antigen
Authors: Townsend, ARM
Bastin, J
Gould, K
Brownlee, GG
Andrew, A
Boyle, DB
Chan, YS
Smith, GL
Item Type: Journal Article
Abstract: Vaccinia infection interferes with the presentation of influenza Haemagglutinin (HA) and Nucleoprotein (NP) to class I-restricted CTL. The inhibitory effect is selective for certain epitopes, and is more profound during the late phase of infection. For influenza A/NT/60/68 NP, the block is present during both early and late phases of infection, and is selective for the COOH-terminal epitope defined by peptide 366-379, having no detectable effect on the presentation of the NH2-terminal epitope 50-63. The presentation of HA is inhibited only during the late phase of vaccinia infection. For both proteins, presentation is partially (NP) or completely (HA) restored by expression of rapidly degraded protein fragments in the vaccinia infected target cell. For HA, deletion of the NH2-terminal signal sequence completely overcomes the block. For NP, either a large NH2-terminal deletion or the construction of a rapidly degraded ubiquitin-NP fusion protein partially restores presentation. These results illustrate the relationship between degradation of viral proteins in the cytoplasm of an infected cell and recognition of epitopes at the cell surface by class I-restricted T cells.
Issue Date: 1-Oct-1988
Date of Acceptance: 1-Oct-1988
URI: http://hdl.handle.net/10044/1/65096
DOI: https://dx.doi.org/10.1084/jem.168.4.1211
ISSN: 0022-1007
Publisher: Rockefeller University Press
Start Page: 1211
End Page: 1224
Journal / Book Title: Journal of Experimental Medicine
Volume: 168
Issue: 4
Copyright Statement: © 1988 Rockefeller University Press.
Keywords: Animals
Cells, Cultured
Epitopes
Female
Genetic Vectors
Hemagglutinins, Viral
Histocompatibility Antigens Class I
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Nucleoproteins
Orthomyxoviridae
Peptide Fragments
Precipitin Tests
Promoter Regions, Genetic
Protein Sorting Signals
Recombinant Fusion Proteins
Recombinant Proteins
T-Lymphocytes, Cytotoxic
Vaccinia virus
11 Medical And Health Sciences
Immunology
Publication Status: Published
Appears in Collections:Department of Medicine
Faculty of Medicine



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