An improved rapid sampling microdialysis system for human and porcine organ monitoring in a hospital setting

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Title: An improved rapid sampling microdialysis system for human and porcine organ monitoring in a hospital setting
Authors: Gowers, S
Hamaoui, K
Vallant, N
Hanna, G
Darzi, A
Casanova, D
Papalois, V
Boutelle, M
Item Type: Journal Article
Abstract: Online organ monitoring could provide clinicians with critical information regarding organ health prior to transplantation and could aid clinical decision-making. This paper presents the methodology of online microdialysis for real-time monitoring of human organs ex vivo. We describe how rapid sampling microdialysis can be incorporated with organ perfusion machines to create a robust organ monitoring system and demonstrate its use in monitoring human and porcine kidneys as well as human and porcine pancreases. In this paper we also show the potential usefulness of this methodology for evaluating novel interventions in a research setting. The analysis system can be configured either to analyse two analytes in one organ, allowing for ratiometric analysis, or alternatively to monitor one analyte in two organs simultaneously, allowing direct comparison. It was found to be reliable over long monitoring periods in real clinical use. The results clearly show that the analysis system is sensitive to differences between organs and therefore has huge potential as an ex vivo organ monitoring tool.
Issue Date: 5-Nov-2018
Date of Acceptance: 22-Sep-2018
URI: http://hdl.handle.net/10044/1/64996
DOI: https://dx.doi.org/10.1039/c8ay01807c
ISSN: 1759-9660
Publisher: Royal Society of Chemistry
Start Page: 5273
End Page: 5281
Journal / Book Title: Analytical Methods
Volume: 10
Issue: 44
Copyright Statement: © 2018 The Author(s). This an open access article licensed under a Creative Commons Attribution 3.0 Unported license (CC BY 3.0), https://creativecommons.org/licenses/by/3.0/
Sponsor/Funder: Imperial College Healthcare NHS Trust- BRC Funding
Wellcome Trust
Funder's Grant Number: RD207
094912/Z/10/Z
Keywords: Science & Technology
Physical Sciences
Life Sciences & Biomedicine
Technology
Chemistry, Analytical
Food Science & Technology
Spectroscopy
Chemistry
ONLINE MICRODIALYSIS
MICROCHIP ELECTROPHORESIS
BRAIN METABOLISM
KIDNEY
PERFUSION
PRESERVATION
BIOSENSORS
SUBSTRATE
ISCHEMIA
SURVIVAL
0301 Analytical Chemistry
0399 Other Chemical Sciences
Publication Status: Published
Appears in Collections:Bioengineering
Division of Surgery
Faculty of Medicine



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