Sparks et al, Heterogeneity in tumor chromatin-doxorubicin binding revealed by in vivo fluorescence lifetime imaging confocal endomicroscopy: in vivo data

Title: Sparks et al, Heterogeneity in tumor chromatin-doxorubicin binding revealed by in vivo fluorescence lifetime imaging confocal endomicroscopy: in vivo data
Authors: Sparks, H
Hooper, S
Sahai, E
Item Type: Dataset
Abstract: Murine xenografts were prepared by intraperitoneal (IP) injection of IGROV-1 cancer cells. IGROV-1 cells were grown to 80% confluence before being trypsinized and re‑suspended in PBS at a concentration of cells per ml. cells were injected into ICRF nude mice. After 14 days post-injection, the presence of intraperitoneal tumors was confirmed by bioluminescence imaging. Briefly, an IVIS bioluminescence imaging system was used to image isoflurane anesthetized mice. 100 µl of D-luciferin (luciferase substrate) at 30mg ml-1 was injected IP 10 minutes before recording of bioluminescence images. The presence of peritoneal tumors was confirmed if bioluminescence signals from the peritoneum were above background noise 10-30 minutes after D‑luciferin injections. Following confirmation of tumors, in vivo fluorescence imaging experiments were carried out after 21 days. To study differences in drug uptake between intravenous or intraperitoneal delivery, prior to imaging mice were subject to IP or IV doxorubicin-based chemotherapy for 1.5, 3 or 24 hours. Imaging involved terminal procedures, mice were anesthetized then peritoneal tumors were exposed by minor surgery and inspected with the CEM. All animal model procedures were approved by The Francis Crick Institute Biological Ethics Committee and UK Home Office authority provided by Project License 70/8380.
Murine xenografts were prepared by intraperitoneal (IP) injection of IGROV-1 cancer cells. IGROV-1 cells were grown to 80% confluence before being trypsinized and re‑suspended in PBS at a concentration of cells per ml. cells were injected into ICRF nude mice. After 14 days post-injection, the presence of intraperitoneal tumors was confirmed by bioluminescence imaging. Briefly, an IVIS bioluminescence imaging system was used to image isoflurane anesthetized mice. 100 µl of D-luciferin (luciferase substrate) at 30mg ml-1 was injected IP 10 minutes before recording of bioluminescence images. The presence of peritoneal tumors was confirmed if bioluminescence signals from the peritoneum were above background noise 10-30 minutes after D‑luciferin injections. Following confirmation of tumors, in vivo fluorescence imaging experiments were carried out after 21 days. To study differences in drug uptake between intravenous or intraperitoneal delivery, prior to imaging mice were subject to IP or IV doxorubicin-based chemotherapy for 1.5, 3 or 24 hours. Imaging involved terminal procedures, mice were anesthetized then peritoneal tumors were exposed by minor surgery and inspected with the CEM. All animal model procedures were approved by The Francis Crick Institute Biological Ethics Committee and UK Home Office authority provided by Project License 70/8380.
Issue Date: 17-May-2018
Citation: 10.1038/s41467-018-04820-6
URI: http://hdl.handle.net/10044/1/64498
DOI: https://doi.org/10.5281/zenodo.1249023
Copyright Statement: https://creativecommons.org/licenses/by/4.0/
Keywords: tumor chromatin-doxorubicin binding
in vivo fluorescence
lifetime imaging
confocal endomicroscopy
in vivo data
Notes: Data is divided into three folders: Sparks_et_al_FIG_6_IP_intranodule_heterogeneity data for Sparks et al Figure 6 main text section: 'FRET between chromatin-bound GFP and doxorubicin' Sparks_et_al_FIG4_5_6_IP_IV_chemo_comparison data for Sparks et al Figures 4,5 & 6 main text section: 'FLIM endomicroscope can monitor doxorubicin cellular uptake' Sparks_et_al_FIG6_IP__internodule_heterogeneity data for Sparks et al Figure 6 main text section: 'Intra-tumor heterogeneity' In vivo experiments
Appears in Collections:Faculty of Natural Sciences - Research Data



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