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Mutualism between Klebsiella SGM 81 and Dianthus caryophyllus in modulating root plasticity and rhizospheric bacterial density

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Title: Mutualism between Klebsiella SGM 81 and Dianthus caryophyllus in modulating root plasticity and rhizospheric bacterial density
Authors: Gang, S
Sarah, M
Waite, C
Buck, M
Schumacher, J
Item Type: Journal Article
Abstract: Aims Dianthus caryophyllus is a commercially important ornamental flower. Plant growth promoting rhizobacteria are increasingly applied as bio-fertilisers and bio-fortifiers. We studied the effect of a rhizospheric isolate Klebsiella SGM 81 strain to promote D. caryophyllus growth under sterile and non-sterile conditions, to colonise its root system endophytically and its impact on the cultivatable microbial community. We identified the auxin indole-3-acetic acid (IAA) production of Klebsiella SGM 81 as major bacterial trait most likely to enhance growth of D. caryophyllus. Methods ipdC dependent IAA production of SGM 81 was quantified using LC-MS/MS and localised proximal to D. caryophyllus roots and correlated to root growth promotion and characteristic morphological changes. SGM 81 cells were localised on and within the plant root using 3D rendering confocal microscopy of gfp expressing SGM 81. Using Salkowski reagent IAA production was quantified and localised proximal to roots in situ. The effect of different bacterial titres on rhizosphere bacterial population was CFU enumerated on nutrient agar. The genome sequence of Klebsiella SGM 81 (accession number PRJEB21197) was determined to validate PGP traits and phylogenic relationships. Results Inoculation of D. caryophyllus roots with Klebsiella SGM 81 drastically promoted plant growth when grown in agar and soil, concomitant with a burst in root hair formation, suggesting an increase in root auxin activity. We sequenced the Klebsiella SGM 81 genome, identified the presence of a canonical ipdC gene in Klebsiella SGM 81, confirmed bacterial production and secretion of IAA in batch culture using LC-MS/MS and localised plant dependent IAA production by SGM 81 proximal to roots. We found Klebsiella SGM 81 to be a rhizoplane and endophytic coloniser of D. caryophyllus roots in a dose dependent manner. We found no adverse effects of SGM 81 on the overall rhizospheric microbial population unless supplied to soil in very high titres. Conclusion Klebsiella SGM 81 effectively improves root traits of D. caryophyllus in a dose dependent manner, likely through tryptophan dependent IAA production in the rhizoplane and potentially within the intercellular spaces of root tissue. Under optimal plant growth promoting conditions in non-sterile soil, the high total microbial titre in the rhizosphere supports a mutualistic relationship between Klebsiella SGM 81 and carnation that potentially extends to the wider rhizosphere microbiota.
Issue Date: 1-Mar-2018
Date of Acceptance: 7-Nov-2017
URI: http://hdl.handle.net/10044/1/64166
DOI: https://dx.doi.org/10.1007/s11104-017-3440-5
ISSN: 0032-079X
Publisher: Springer Verlag
Start Page: 273
End Page: 288
Journal / Book Title: Plant and Soil
Volume: 424
Copyright Statement: © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/GCRF-IAA/17/10
Keywords: Science & Technology
Life Sciences & Biomedicine
Agronomy
Plant Sciences
Soil Science
Agriculture
Dianthus caryophyllus
IAA
Klebsiella
Plant inoculation assays
Root plasticity
GROWTH-PROMOTING RHIZOBACTERIA
ZEA-MAYS L
PLANT-GROWTH
PSEUDOMONAS-PUTIDA
WILD-TYPE
TRITICUM-AESTIVUM
NITROGEN-FIXATION
INDOLEACETIC-ACID
MOLECULAR-CLONING
JATROPHA-CURCAS
05 Environmental Sciences
06 Biological Sciences
07 Agricultural And Veterinary Sciences
Agronomy & Agriculture
Publication Status: Published
Online Publication Date: 2017-11-08
Appears in Collections:Faculty of Natural Sciences



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