Altmetric

Rapid prototyping platform for Saccharomyces cerevisiae using computer-aided genetic design enabled by parallel software and workcell platform development

File Description SizeFormat 
Main_text.docxAccepted version45.19 kBMicrosoft WordView/Open
Title: Rapid prototyping platform for Saccharomyces cerevisiae using computer-aided genetic design enabled by parallel software and workcell platform development
Authors: Rajakumar, PD
Gower, G
Suckling, L
Kitney, R
McClymont, D
Freemont, P
Item Type: Journal Article
Abstract: Biofoundries have enabled the ability to automate the construction of genetic constructs using computer-aided design. In this study, we have developed the methodology required to abstract and automate the construction of yeast-compatible designs. We demonstrate the use of our in-house software tool, AMOS, to coordinate with design software, JMP, and robotic liquid handling platforms to successfully manage the construction of a library of 88 yeast expression plasmids. In this proof-of-principle study, we used three fluorescent genes as proxy for three enzyme coding sequences. Our platform has been designed to quickly iterate around a design cycle of four protein coding sequences per plasmid, with larger numbers possible with multiplexed genome integrations in Saccharomyces cerevisiae. This work highlights how developing scalable new biotechnology applications requires a close integration between software development, liquid handling robotics, and protocol development.
Issue Date: 30-Aug-2018
Date of Acceptance: 10-Aug-2018
URI: http://hdl.handle.net/10044/1/63436
DOI: https://dx.doi.org/10.1177/2472630318798304
ISSN: 2472-6303
Publisher: SAGE Publications
Journal / Book Title: Slas Technology
Copyright Statement: © 2018 Society for Laboratory Automation and Screening. The final, definitive version of this paper has been published in Slas Technology by Sage Publications Ltd. All rights reserved. It is available at: http://journals.sagepub.com/doi/10.1177/2472630318798304
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/K006290/1
BB/K019791/1
BB/L027852/1
Keywords: acoustic liquid handling workcell
design of eperiment (DOE)
high-throughput yeast transformation
synthetic biology
yeast toolkit (YTK)—Golden Gate
Publication Status: Published online
Online Publication Date: 2018-08-30
Appears in Collections:Faculty of Engineering
Bioengineering
Department of Medicine
Faculty of Medicine



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commonsx