Non-aqueous homogenous biocatalytic conversion of polysaccharides in ionic liquids using chemically modified glucosidase

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Title: Non-aqueous homogenous biocatalytic conversion of polysaccharides in ionic liquids using chemically modified glucosidase
Authors: Brogan, APS
Bui-Le, L
Hallett, JP
Item Type: Journal Article
Publication Date: 2018-12-25T07:00:26Z
Abstract: The increasing requirement to produce platform chemicals and fuels from renewable sources means advances in biocatalysis are rapidly becoming a necessity. Biomass is widely used in nature as a source of energy and as chemical building blocks. However, recalcitrance towards traditional chemical processes and solvents provides a significant barrier to widespread utility. Here, by optimizing enzyme solubility in ionic liquids, we have discovered solvent-induced substrate promiscuity of glucosidase, demonstrating an unprecedented example of homogeneous enzyme bioprocessing of cellulose. Specifically, chemical modification of glucosidase for solubilization in ionic liquids can increase thermal stability to up to 137 °C, allowing for enzymatic activity 30 times greater than is possible in aqueous media. These results establish that through a synergistic combination of chemical biology (enzyme modification) and reaction engineering (solvent choice), the biocatalytic capability of enzymes can be intensified: a key step towards the full-scale deployment of industrial biocatalysis.
Issue Date: 1-Aug-2018
Date of Acceptance: 23-May-2018
URI: http://hdl.handle.net/10044/1/62242
DOI: https://dx.doi.org/10.1038/s41557-018-0088-6
ISSN: 1755-4330
Publisher: Nature Publishing Group
Start Page: 859
End Page: 865
Journal / Book Title: Nature Chemistry
Volume: 10
Issue: 8
Copyright Statement: © 2018, Springer Nature.
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
CIRCULAR-DICHROISM SPECTROSCOPY
SECONDARY STRUCTURE ANALYSES
ORGANIC-SOLVENTS
BIOMASS
ENZYMES
DECONSTRUCTION
NANOCONSTRUCTS
MYOGLOBIN
BIOFUELS
WATER
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
CIRCULAR-DICHROISM SPECTROSCOPY
SECONDARY STRUCTURE ANALYSES
ORGANIC-SOLVENTS
BIOMASS
ENZYMES
DECONSTRUCTION
NANOCONSTRUCTS
MYOGLOBIN
BIOFUELS
WATER
03 Chemical Sciences
Organic Chemistry
Publication Status: Published
metadata.pubs.date.publish-online: 2018-06-25
Appears in Collections:Faculty of Engineering
Chemistry
Chemical Engineering
Faculty of Natural Sciences



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