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A novel In vitro model of blast traumatic brain injury

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Title: A novel In vitro model of blast traumatic brain injury
Authors: Campos Pires, R
Yonis, A
Macdonald, W
Harris, K
Edge, C
Mahoney, P
Dickinson, R
Item Type: Journal Article
Abstract: Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the detonation of explosive devices, however, the mechanisms that underlie the brain damage resulting from blast overpressure exposure are not entirely understood and are believed to be unique to this type of brain injury. Preclinical models are crucial tools that contribute to better understand blast-induced brain injury. A novel in vitro blast TBI model was developed using an open-ended shock tube to simulate real-life open-field blast waves modelled by the Friedlander waveform. C57BL/6N mouse organotypic hippocampal slice cultures were exposed to single shock waves and the development of injury was characterized up to 72 h using propidium iodide, a well-established fluorescent marker of cell damage that only penetrates cells with compromised cellular membranes. Propidium iodide fluorescence was significantly higher in the slices exposed to a blast wave when compared with sham slices throughout the duration of the protocol. The brain tissue injury is very reproducible and proportional to the peak overpressure of the shock wave applied.
Issue Date: 21-Dec-2018
Date of Acceptance: 5-Jul-2018
URI: http://hdl.handle.net/10044/1/62079
DOI: https://dx.doi.org/10.3791/58400
ISSN: 1940-087X
Publisher: Journal of Visualized Experiments
Journal / Book Title: Jove-Journal of Visualized Experiments
Volume: 142
Copyright Statement: © 2018 The Author(s). This is an open access article distributred under the terms of the Creative Commons Attribution 3.0 License (https://creativecommons.org/licenses/by/3.0/)
Sponsor/Funder: Westminster Medical School Research Trust
Royal Centre for Defence Medicine
The Royal British Legion
Medical Research Council (MRC)
Medical Research Council (MRC)
The Gas Safety Trust
Royal Centre for Defence Medicine
Funder's Grant Number: JRC 1-/11 PHD 001
20120229-DMSRASG/Xenon
Centre for Blast Injury Studie
MC_PC_13064
MR/N027736/1
WSSA_P64107
1012/4
Publication Status: Published
Open Access location: https://www.jove.com/video/58400/a-novel-in-vitro-model-of-blast-traumatic-brain-injury
Article Number: e58400
Online Publication Date: 2018-12-21
Appears in Collections:Division of Surgery
Faculty of Medicine



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