Why the COI barcode should be the community DNA metabarcode for the Metazoa

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Title: Why the COI barcode should be the community DNA metabarcode for the Metazoa
Author(s): Andújar, C
Arribas, P
Yu, DW
Vogler, AP
Emerson, BC
Item Type: Journal Article
Abstract: Metabarcoding of complex metazoan communities is increasingly being used to measure biodiversity in terrestrial, freshwater, and marine ecosystems, revolutionizing our ability to observe patterns and infer processes regarding the origin and conservation of biodiversity. A fundamentally important question is which genetic marker to amplify, and although the mitochondrial cytochrome oxidase subunit I (COI) gene is one of the more widely used markers in metabarcoding for the Metazoa, doubts have recently been raised about its suitability. We argue that (i) the extensive coverage of reference-sequence databases for COI, (ii) the variation it presents, (iii) the comparative advantages for denoising protein coding genes, and (iv) recent advances in DNA sequencing protocols argue in favour of standardising for the use of COI for metazoan community samples. We also highlight where research efforts should focus to maximise the utility of metabarcoding. This article is protected by copyright. All rights reserved.
Publication Date: 21-Aug-2018
Date of Acceptance: 1-Aug-2018
URI: http://hdl.handle.net/10044/1/61906
DOI: https://dx.doi.org/10.1111/mec.14844
ISSN: 0962-1083
Publisher: Wiley
Journal / Book Title: Molecular Ecology
Copyright Statement: © 2018 Wiley. This is the accepted version of an article which has been published in final form at https://dx.doi.org/10.1111/mec.14844
Keywords: eDNA
High Throughput Sequencing (HTS)
Metabarcoding
Next Generation Sequencing (NGS)
barcoding
eDNA
High Throughput Sequencing (HTS)
Metabarcoding
Next Generation Sequencing (NGS)
barcoding
06 Biological Sciences
Evolutionary Biology
Publication Status: Published online
Conference Place: England
Embargo Date: 2019-08-21
Online Publication Date: 2018-08-21
Appears in Collections:Faculty of Natural Sciences



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