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Metabolic phenotyping and strain characterisation of pseudomonas aeruginosa Isolates from cystic fibrosis patients using rapid evaporative ionisation mass spectrometry

Title: Metabolic phenotyping and strain characterisation of pseudomonas aeruginosa Isolates from cystic fibrosis patients using rapid evaporative ionisation mass spectrometry
Authors: Bardin, EE
Cameron, SJS
Perdones-Montero, A
Hardiman, K
Bolt, F
Alton, EWFW
Bush, A
Davies, JC
Takats, Z
Item Type: Journal Article
Abstract: Rapid evaporative ionisation mass spectrometry (REIMS) is a novel technique for the real-time analysis of biological material. It works by conducting an electrical current through a sample, causing it to rapidly heat and evaporate, with the analyte containing vapour channelled to a mass spectrometer. It was used to characterise the metabolome of 45 Pseudomonas aeruginosa (P. aeruginosa) isolates from cystic fibrosis (CF) patients and compared to 80 non-CF P. aeruginosa. Phospholipids gave the highest signal intensity; 17 rhamnolipids and 18 quorum sensing molecules were detected, demonstrating that REIMS has potential for the study of virulence-related metabolites. P. aeruginosa isolates obtained from respiratory samples showed a higher diversity, which was attributed to the chronic nature of most respiratory infections. The analytical sensitivity of REIMS allowed the detection of a metabolome that could be used to classify individual P. aeruginosa isolates after repeated culturing with 81% accuracy, and an average 83% concordance with multilocus sequence typing. This study underpins the capacities of REIMS as a tool with clinical applications, such as metabolic phenotyping of the important CF pathogen P. aeruginosa, and highlights the potential of metabolic fingerprinting for fine scale characterisation at a sub-species level.
Issue Date: 19-Jul-2018
Date of Acceptance: 22-Jun-2018
URI: http://hdl.handle.net/10044/1/61707
DOI: https://dx.doi.org/10.1038/s41598-018-28665-7
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 8
Copyright Statement: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre- ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per- mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Sponsor/Funder: Cystic Fibrosis Trust
Funder's Grant Number: SRC 001
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
P. AERUGINOSA
OPPORTUNISTIC PATHOGEN
QUINOLONE SIGNAL
MALDI
TIME
RHAMNOLIPIDS
SEQUENCE
IDENTIFICATION
MECHANISMS
DIVERSITY
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
P. AERUGINOSA
OPPORTUNISTIC PATHOGEN
QUINOLONE SIGNAL
MALDI
TIME
RHAMNOLIPIDS
SEQUENCE
IDENTIFICATION
MECHANISMS
DIVERSITY
Publication Status: Published
Article Number: ARTN 10952
Appears in Collections:Division of Surgery
National Heart and Lung Institute
Faculty of Medicine



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