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A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing.

Title: A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing.
Authors: Daly, GM
Bexfield, N
Heaney, J
Stubbs, S
Mayer, AP
Palser, A
Kellam, P
Drou, N
Caccamo, M
Tiley, L
Alexander, GJM
Bernal, W
Heeney, JL
Item Type: Journal Article
Abstract: Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.
Issue Date: 21-Dec-2011
Date of Acceptance: 16-Nov-2011
URI: http://hdl.handle.net/10044/1/61516
DOI: https://dx.doi.org/10.1371/journal.pone.0028879
ISSN: 1932-6203
Publisher: Public Library of Science
Start Page: e28879
End Page: e28879
Journal / Book Title: PLoS One
Volume: 6
Issue: 12
Copyright Statement: © 2011 Daly et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Animals
Biopsy
Dogs
Humans
Liver
RNA, Viral
Viruses
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Conference Place: United States
Article Number: e28879
Appears in Collections:Department of Medicine
Faculty of Medicine



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