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Ycf48 involved in the biogenesis of the oxygen-evolving photosystem II complex is a seven-bladed beta-propeller protein

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Title: Ycf48 involved in the biogenesis of the oxygen-evolving photosystem II complex is a seven-bladed beta-propeller protein
Authors: Yu, J
Knoppova, J
Michoux, F
Bialek, W
Cota Segura, E
Shukla, M
Straskova, A
Aznar, G
Sobotka, R
Komenda, J
Murray, J
Nixon, PJ
Item Type: Journal Article
Abstract: Robust photosynthesis in chloroplasts and cyanobacteria requires the participation of accessory proteins to facilitate the assembly and maintenance of the photosynthetic apparatus located within the thylakoid membranes. The highly conserved Ycf48 protein acts early in the biogenesis of the oxygen-evolving photosystem II (PSII) complex by binding to newly synthesized precursor D1 subunit and by promoting efficient association with the D2 protein to form a PSII reaction center (PSII RC) assembly intermediate. Ycf48 is also required for efficient replacement of damaged D1 during the repair of PSII. However, the structural features underpinning Ycf48 function remain unclear. Here we show that Ycf48 proteins encoded by the thermophilic cyanobacterium Thermosynechococcus elongatus and the red alga Cyanidioschyzon merolae form seven-bladed beta-propellers with the 19-aa insertion characteristic of eukaryotic Ycf48 located at the junction of blades 3 and 4. Knowledge of these structures has allowed us to identify a conserved “Arg patch” on the surface of Ycf48 that is important for binding of Ycf48 to PSII RCs but also to larger complexes, including trimeric photosystem I (PSI). Reduced accumulation of chlorophyll in the absence of Ycf48 and the association of Ycf48 with PSI provide evidence of a more wide-ranging role for Ycf48 in the biogenesis of the photosynthetic apparatus than previously thought. Copurification of Ycf48 with the cyanobacterial YidC protein insertase supports the involvement of Ycf48 during the cotranslational insertion of chlorophyll-binding apopolypeptides into the membrane.
Issue Date: 14-Aug-2018
Date of Acceptance: 15-Jun-2018
URI: http://hdl.handle.net/10044/1/61495
DOI: https://dx.doi.org/10.1073/pnas.1800609115
ISSN: 0027-8424
Publisher: National Academy of Sciences
Start Page: E7824
End Page: E7833
Journal / Book Title: Proceedings of the National Academy of Sciences
Volume: 115
Issue: 33
Copyright Statement: © 2018 The Author(s). Published under the PNAS license (http://www.pnas.org/page/authors/licenses)
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/I00937X/1
BB/L003260/1
BB/F023308/1
BB/P00931X/1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
photosystem II
photosynthesis
chlorophyll-binding proteins
SP PCC 6803
SYNECHOCYSTIS SP PCC-6803
ARABIDOPSIS-THALIANA
WATER OXIDATION
EARLY STEPS
HIGH LIGHT
CHLOROPHYLL
REPAIR
CYANOBACTERIA
HOMOLOG
chlorophyll-binding proteins
photosynthesis
photosystem II
Bacterial Proteins
Cyanobacteria
Photosystem I Protein Complex
Photosystem II Protein Complex
Cyanobacteria
Photosystem I Protein Complex
Photosystem II Protein Complex
Bacterial Proteins
MD Multidisciplinary
Publication Status: Published
Online Publication Date: 2018-07-30
Appears in Collections:Faculty of Natural Sciences



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