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Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection.

Title: Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection.
Authors: Garson, JA
Kellam, P
Towers, GJ
Item Type: Journal Article
Abstract: XMRV is a gammaretrovirus associated in some studies with human prostate cancer and chronic fatigue syndrome. Central to the hypothesis of XMRV as a human pathogen is the description of integration sites in DNA from prostate tumour tissues. Here we demonstrate that 2 of 14 patient-derived sites are identical to sites cloned in the same laboratory from experimentally infected DU145 cells. Identical integration sites have never previously been described in any retrovirus infection. We propose that the patient-derived sites are the result of PCR contamination. This observation further undermines the notion that XMRV is a genuine human pathogen.
Issue Date: 25-Feb-2011
Date of Acceptance: 25-Feb-2011
URI: http://hdl.handle.net/10044/1/61384
DOI: https://dx.doi.org/10.1186/1742-4690-8-13
ISSN: 1742-4690
Publisher: BioMed Central
Start Page: 13
End Page: 13
Journal / Book Title: Retrovirology
Volume: 8
Issue: 13
Copyright Statement: © 2011 Garson et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Base Sequence
Cell Line, Tumor
DNA Contamination
DNA, Viral
Humans
Male
Molecular Sequence Data
Polymerase Chain Reaction
Prostate
Prostatic Neoplasms
Virus Integration
Xenotropic murine leukemia virus-related virus
1103 Clinical Sciences
Virology
Publication Status: Published online
Conference Place: England
Appears in Collections:Department of Medicine
Faculty of Medicine



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