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No evidence of XMRV or related retroviruses in a London HIV-1-positive patient cohort.

Title: No evidence of XMRV or related retroviruses in a London HIV-1-positive patient cohort.
Authors: Gray, ER
Garson, JA
Breuer, J
Edwards, S
Kellam, P
Pillay, D
Towers, GJ
Item Type: Journal Article
Abstract: BACKGROUND: Several studies have implicated a recently discovered gammaretrovirus, XMRV (Xenotropic murine leukaemia virus-related virus), in chronic fatigue syndrome and prostate cancer, though whether as causative agent or opportunistic infection is unclear. It has also been suggested that the virus can be found circulating amongst the general population. The discovery has been controversial, with conflicting results from attempts to reproduce the original studies. METHODOLOGY/PRINCIPAL FINDINGS: We extracted peripheral blood DNA from a cohort of 540 HIV-1-positive patients (approximately 20% of whom have never been on anti-retroviral treatment) and determined the presence of XMRV and related viruses using TaqMan PCR. While we were able to amplify as few as 5 copies of positive control DNA, we did not find any positive samples in the patient cohort. CONCLUSIONS/SIGNIFICANCE: In view of these negative findings in this highly susceptible group, we conclude that it is unlikely that XMRV or related viruses are circulating at a significant level, if at all, in HIV-1-positive patients in London or in the general population.
Issue Date: 23-Mar-2011
Date of Acceptance: 21-Feb-2011
URI: http://hdl.handle.net/10044/1/61367
DOI: https://dx.doi.org/10.1371/journal.pone.0018096
ISSN: 1932-6203
Publisher: Public Library of Science
Start Page: e18096
End Page: e18096
Journal / Book Title: PLoS One
Volume: 6
Issue: 3
Copyright Statement: © 2011 Gray et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Adolescent
Adult
Aged
Aged, 80 and over
Cohort Studies
DNA
Female
Genome, Viral
HIV Seropositivity
HIV-1
Humans
Leukocytes
London
Male
Middle Aged
Polymerase Chain Reaction
Xenotropic murine leukemia virus-related virus
Young Adult
MD Multidisciplinary
General Science & Technology
Publication Status: Published online
Conference Place: United States
Article Number: e18096
Appears in Collections:Department of Medicine
Faculty of Medicine



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