Probing synaptic amyloid-beta aggregation promoted by copper release

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Title: Probing synaptic amyloid-beta aggregation promoted by copper release
Authors: Tahirbegi, B
Magness, AJ
Boillat, A
Willison, KR
Klug, DR
Knopfel, T
Ying, L
Item Type: Conference Paper
Abstract: Whether or not the metal ions released during synaptic transmission induce amyloid-beta oligomer formation in the vicinity of synapses is a central question pertinent to the molecular mechanism of Alzheimer's disease. Recently, through a combination of experimental kinetics studies and coupled reaction-diffusion simulations, we predicted that Cu(II) rather than Zn(II) plays an important role in the very early stages (i.e., dimer formation) of Aβ aggregation in the synapse. Single molecule photobleaching analysis is a powerful tool to determine the stoichiometry of amyloid-beta oligomers which enables us to examine the time course of small amyloid-beta oligomer formation in solution, immobilised to a solid-phase substrate or artificial lipid membrane, and in live neurons in the presence of Cu(II). Preliminary results indicate that small amyloid-beta oligomers can be locked in their oligomeric state without dissociation on a poly-lysine coated surface and that Cu(II) increases the diversity and abundance of amyloid-beta oligomers.
Issue Date: 2-Feb-2018
Date of Acceptance: 6-Oct-2017
URI: http://hdl.handle.net/10044/1/60796
DOI: https://dx.doi.org/10.1016/j.bpj.2017.11.2383
ISSN: 0006-3495
Publisher: Biophysical Society
Start Page: 430A
End Page: 430A
Journal / Book Title: Biophysical Journal
Volume: 114
Issue: 3, Supplement 1
Copyright Statement: © 2018 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Conference Name: 62nd Annual Meeting of the Biophysical-Society
Keywords: Science & Technology
Life Sciences & Biomedicine
Biophysics
02 Physical Sciences
03 Chemical Sciences
06 Biological Sciences
Publication Status: Published
Start Date: 2018-02-17
Finish Date: 2018-02-21
Conference Place: San Francisco, CA
Online Publication Date: 2018-02-06
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
National Heart and Lung Institute
Department of Medicine
Faculty of Medicine
Faculty of Natural Sciences



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