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Insights into the evolution of the bacterial flagellar motor from highthroughput in situ electron cryotomography and subtomogram averaging

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Title: Insights into the evolution of the bacterial flagellar motor from highthroughput in situ electron cryotomography and subtomogram averaging
Authors: Rossmann, F
Beeby, M
Item Type: Journal Article
Abstract: In situ structural information on molecular machines can be invaluable in understanding their assembly, mechanism and evolution. Here, the use of electron cryotomography (ECT) to obtain significant insights into how an archetypal molecular machine, the bacterial flagellar motor, functions and how it has evolved is described. Over the last decade, studies using a high-throughput, medium-resolution ECT approach combined with genetics, phylogenetic reconstruction and phenotypic analysis have revealed surprising structural diversity in flagellar motors. Variations in the size and the number of torque-generating proteins in the motor visualized for the first time using ECT has shown that these variations have enabled bacteria to adapt their swimming torque to the environment. Much of the structural diversity can be explained in terms of scaffold structures that facilitate the incorporation of additional motor proteins, and more recent studies have begun to infer evolutionary pathways to higher torque-producing motors. This review seeks to highlight how the emerging power of ECT has enabled the inference of ancestral states from various bacterial species towards understanding how, and `why', flagellar motors have evolved from an ancestral motor to a diversity of variants with adapted or modified functions.
Issue Date: 1-Jun-2018
Date of Acceptance: 29-May-2018
URI: http://hdl.handle.net/10044/1/60449
DOI: https://dx.doi.org/10.1107/S2059798318007945
ISSN: 1399-0047
Publisher: International Union of Crystallography
Start Page: 585
End Page: 594
Journal / Book Title: Acta Crystallographica Section D: Biological Crystallography
Volume: D74
Copyright Statement: © 2018 The Author(s). This article is available under a CC-BY Licence (https://creativecommons.org/licenses/by/2.0/uk/legalcode)
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/L023091/1
Keywords: bacterial flagellar motors
electron cryotomography
low-abundance imaging
molecular evolution
subtomogram averaging
Publication Status: Published
Appears in Collections:Faculty of Natural Sciences



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