Cross-contamination explains "inter- and intraspecific horizontal genetic transfers" between asexual bdelloid rotifers

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Title: Cross-contamination explains "inter- and intraspecific horizontal genetic transfers" between asexual bdelloid rotifers
Author(s): Wilson, CG
Barraclough, T
Nowell, R
Item Type: Journal Article
Abstract: A few metazoan lineages are thought to have persisted for millions of years without sexual reproduction. If so, they would offer important clues to the evolutionary paradox of sex itself [1, 2]. Most "ancient asexuals" are subject to ongoing doubt because extant populations continue to invest 17 in males [3–9]. However, males are famously unknown in bdelloid rotifers, a class of microscopic invertebrates comprising hundreds of species [10–12]. Bdelloid genomes have acquired an unusually high proportion of genes from non-metazoans via horizontal transfer [13–17]. This well-substantiated finding has invited speculation [13] that homologous horizontal transfer between bdelloid individuals also may occur, perhaps even "replacing" sex [14]. In 2016, Current Biology published an Article claiming to supply evidence for this idea. Debortoli et al. [18] sampled rotifers from natural populations and sequenced one mitochondrial and four nuclear loci. Species assignments were incongruent among loci for several samples, which was interpreted as evidence of "interspecific horizontal genetic transfers". Here, we use sequencing chromatograms supplied by the authors to demonstrate that samples treated as individuals actually contained two or more highly divergent mitoc hondrial and ribosomal sequences, revealing cross-contamination with DNA from multiple animals of different species. Other chromatograms indicate contamination with DNA from conspecific animals, explaining genetic and genomic evidence for "intraspecific horizontal exchanges" reported in the same study. Given the clear evidence of contamination, the data and findings of Debortoli et al. [18] provide no reliable support for their conclusions that DNA is transferred horizontally between or within bdelloid species.
Publication Date: 1-Jan-2018
Date of Acceptance: 23-May-2018
URI: http://hdl.handle.net/10044/1/60272
ISSN: 1879-0445
Publisher: Elsevier
Journal / Book Title: Current Biology
Copyright Statement: This paper is embargoed until publication. Once published it will be available fully open access.
Sponsor/Funder: Natural Environment Research Council (NERC)
Natural Environment Research Council (NERC)
Funder's Grant Number: NE/J01933X/1
NE/M01651X/1
Keywords: 06 Biological Sciences
11 Medical And Health Sciences
17 Psychology And Cognitive Sciences
Developmental Biology
Publication Status: Accepted
Embargo Date: publication subject to indefinite embargo
Appears in Collections:Faculty of Natural Sciences



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