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Impact of contusion injury on intramuscular emm1 group A -Streptococcus infection and Lymphatic spread

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Title: Impact of contusion injury on intramuscular emm1 group A -Streptococcus infection and Lymphatic spread
Authors: Lamb, LE
Siggins, MK
Scudamore, C
Macdonald, W
Turner, CE
Lynskey, NN
Tan, LKK
Sriskandan, S
Item Type: Journal Article
Abstract: Invasive group A Streptococcus (iGAS) is frequently associated with emm1 isolates, with an attendant mortality of around 20%. Cases occasionally arise in previously healthy individuals with a history of upper respiratory tract infection, soft tissue contusion, and no obvious portal of entry. Using a new murine model of contusion, we determined the impact of contusion on iGAS bacterial burden and phenotype. Calibrated mild blunt contusion did not provide a focus for initiation or seeding of GAS that was detectable following systemic GAS bacteremia, but instead enhanced GAS migration to the local draining lymph node following GAS inoculation at the same time and site of contusion. Increased migration to lymph node was associated with emergence of mucoid bacteria, although was not specific to mucoid bacteria. In one study, mucoid colonies demonstrated a significant increase in capsular hyaluronan that was not linked to a covRS or rocA mutation, but to a deletion in the promoter of the capsule synthesis locus, hasABC, resulting in a strain with increased fitness for lymph node migration. In summary, in the mild contusion model used, we could not detect seeding of muscle by GAS. Contusion promoted bacterial transit to the local lymph node. The consequences of contusion-associated bacterial lymphatic migration may vary depending on the pathogen and virulence traits selected.
Issue Date: 27-Jul-2018
Date of Acceptance: 22-May-2018
URI: http://hdl.handle.net/10044/1/60256
DOI: https://dx.doi.org/10.1080/21505594.2018.1482180
ISSN: 2150-5594
Publisher: Taylor & Francis
Start Page: 1074
End Page: 1084
Journal / Book Title: Virulence
Volume: 9
Issue: 1
Sponsor/Funder: Royal Army Medical Corps Charity
Medical Research Council (MRC)
Funder's Grant Number: RHQ/34
MR/L008610/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Immunology
Infectious Diseases
Microbiology
Group A streptococcus
contusion
mucoid
lymphatics
capsule
streptococcus pyogenes
hyaluronan
hyaluronic acid
trauma
TOXIC-SHOCK-SYNDROME
NECROTIZING FASCIITIS
MOLECULAR ANALYSIS
PYOGENES
MODEL
CAPSULE
SUFFICIENT
SPYCEP
SKIN
Group A streptococcus
capsule
contusion
hyaluronan
hyaluronic acid
lymphatics
mucoid
streptococcus pyogenes
trauma
Animals
Antigens, Bacterial
Bacterial Outer Membrane Proteins
Carrier Proteins
Contusions
Female
Gene Expression Regulation, Bacterial
Humans
Lymph Nodes
Mice
Muscles
Streptococcal Infections
Streptococcus pyogenes
Virulence
Muscles
Lymph Nodes
Animals
Humans
Mice
Streptococcus pyogenes
Streptococcal Infections
Contusions
Bacterial Outer Membrane Proteins
Carrier Proteins
Antigens, Bacterial
Virulence
Gene Expression Regulation, Bacterial
Female
Publication Status: Published
Online Publication Date: 2018-07-27
Appears in Collections:Department of Medicine
Faculty of Medicine



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