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Engineering thermoresponsive phase separated vesicles formed via emulsion phase transfer as a content-release platform

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Title: Engineering thermoresponsive phase separated vesicles formed via emulsion phase transfer as a content-release platform
Authors: Karamdad, K
Hindley, J
Friddin, MS
Bolognesi, G
Law, RV
Brooks, NJ
Ces, O
Elani, Y
Item Type: Journal Article
Abstract: Giant unilamellar vesicles (GUVs) are a well-established tool for the study of membrane biophysics and are increasingly used as artificial cell models and functional units in biotechnology. This trend is driven by the development of emulsion-based generation methods such as Emulsion Phase Transfer (EPT), which facilitates the encapsulation of almost any water-soluble compounds (including biomolecules) regardless of size or charge, is compatible with droplet microfluidics, and allows GUVs with asymmetric bilayers to be assembled. However, the ability to control the composition of membranes formed via EPT remains an open question; this is key as composition gives rise to an array of biophysical phenomena which can be used to add functionality to membranes. Here, we evaluate the use of GUVs constructed via this method as a platform for phase behaviour studies and take advantage of composition-dependent features to engineer thermally-responsive GUVs. For the first time, we generate ternary GUVs (DOPC/DPPC/cholesterol) using EPT, and by compensating for the lower cholesterol incorporation efficiencies, show that these possess the full range of phase behaviour displayed by electroformed GUVs. As a demonstration of the fine control afforded by this approach, we demonstrate release of dye and peptide cargo when ternary GUVs are heated through the immiscibility transition temperature, and show that release temperature can be tuned by changing vesicle composition. We show that GUVs can be individually addressed and release triggered using a laser beam. Our findings validate EPT as a suitable method for generating phase separated vesicles and provide a valuable proof-of-concept for engineering content release functionality into individually addressable vesicles, which could have a host of applications in the development of smart synthetic biosystems.
Issue Date: 11-May-2018
Date of Acceptance: 22-Apr-2018
URI: http://hdl.handle.net/10044/1/59258
DOI: https://dx.doi.org/10.1039/C7SC04309K
ISSN: 2041-6520
Publisher: Royal Society of Chemistry
Start Page: 4851
End Page: 4858
Journal / Book Title: Chemical Science
Volume: 9
Issue: 21
Copyright Statement: © The Royal Society of Chemistry 2018. is article is licensed under a Creative Commons Attribution 3.0 Unported Licence (https://creativecommons.org/licenses/by/3.0/)
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (E
Funder's Grant Number: EP/N016998/1
EP/J017566/1
EP/G00465X/1
EP/K038648/1
EP/K503733/1
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
GIANT LIPID VESICLES
MEMBRANE-PERMEABILITY
BILAYER-MEMBRANES
ARTIFICIAL CELLS
TEMPERATURE
MICRODROPLETS
FLUCTUATIONS
CONSTRUCTION
CHOLESTEROL
TRANSITION
Publication Status: Published
Open Access location: http://pubs.rsc.org/en/Content/ArticleLanding/2018/SC/C7SC04309K#!divAbstract
Online Publication Date: 2018-05-11
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Faculty of Natural Sciences



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