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Assessment of microbiota:host interactions at the vaginal mucosa interface

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Title: Assessment of microbiota:host interactions at the vaginal mucosa interface
Authors: Pruski, P
Lewis, H
Lee, Y
Marchesi, JR
Bennett, P
Takats, Z
MacIntyre, DA
Item Type: Journal Article
Abstract: There is increasing appreciation of the role that vaginal microbiota play in health and disease throughout a woman’s lifespan. This has been driven partly by molecular techniques that enable detailed identification and characterisation of microbial community structures. However, these methods do not enable assessment of the biochemical and immunological interactions between host and vaginal microbiota involved in pathophysiology. This review examines our current knowledge of the relationships that exist between vaginal microbiota and the host at the level of the vaginal mucosal interface. We also consider methodological approaches to microbiomic, immunologic and metabolic profiling that permit assessment of these interactions. Integration of information derived from these platforms brings the potential for biomarker discovery, disease risk stratification and improved understanding of the mechanisms regulating vaginal microbial community dynamics in health and disease.
Issue Date: 1-Oct-2018
Date of Acceptance: 22-Apr-2018
URI: http://hdl.handle.net/10044/1/59257
DOI: https://dx.doi.org/10.1016/j.ymeth.2018.04.022
ISSN: 1046-2023
Publisher: Elsevier
Start Page: 74
End Page: 84
Journal / Book Title: Methods
Volume: 149
Copyright Statement: © 2018 Elsevier Inc. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Funder's Grant Number: MR/L009226/1
MR/L009226/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
Vagina
Metabolomics
Microbiome
Next generation sequencing
Mass spectrometry
Ambient ionisation
Point-of-care diagnostics
Mucosa
FEMALE GENITAL-TRACT
DESORPTION ELECTROSPRAY-IONIZATION
DIAGNOSING BACTERIAL VAGINOSIS
INNATE IMMUNE-RESPONSE
16S RIBOSOMAL-RNA
MASS-SPECTROMETRY
PREGNANT-WOMEN
NONSPECIFIC VAGINITIS
PRETERM BIRTH
ORGANIC-ACIDS
1103 Clinical Sciences
Publication Status: Published
Online Publication Date: 2018-04-27
Appears in Collections:Division of Surgery
Faculty of Medicine



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