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Normal mitochondrial function in Saccharomyces cerevisiae has become dependent on inefficient splicing

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Title: Normal mitochondrial function in Saccharomyces cerevisiae has become dependent on inefficient splicing
Authors: Rudan, M
Dib, PB
Musa, M
Kanunnikau, M
Sobocanec, S
Rueda, D
Warnecke, T
Krisko, A
Item Type: Journal Article
Abstract: Self-splicing introns are mobile elements that have invaded a number of highly conserved genes in prokaryotic and organellar genomes. Here, we show that deletion of these selfish elements from the Saccharomyces cerevisiae mitochondrial genome is stressful to the host. A strain without mitochondrial introns displays hallmarks of the retrograde response, with altered mitochondrial morphology, gene expression and metabolism impacting growth and lifespan. Deletion of the complete suite of mitochondrial introns is phenocopied by overexpression of the splicing factor Mss116. We show that, in both cases, abnormally efficient transcript maturation results in excess levels of mature cob and cox1 host mRNA. Thus, inefficient splicing has become an integral part of normal mitochondrial gene expression. We propose that the persistence of S. cerevisiae self-splicing introns has been facilitated by an evolutionary lock-in event, where the host genome adapted to primordial invasion in a way that incidentally rendered subsequent intron loss deleterious.
Issue Date: 23-Mar-2018
Date of Acceptance: 19-Mar-2018
URI: http://hdl.handle.net/10044/1/59155
DOI: https://dx.doi.org/10.7554/eLife.35330
ISSN: 2050-084X
Publisher: eLife Sciences Publications Ltd
Journal / Book Title: eLife
Volume: 7
Copyright Statement: © Rudan et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Sponsor/Funder: Medical Research Council
Funder's Grant Number: MC_UP_1102/5
Keywords: Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
SELFISH GENETIC ELEMENTS
PUTATIVE RNA HELICASE
DEAD-BOX PROTEIN
GROUP-I
YEAST
INTRONS
TRANSFORMATION
DISRUPTION
MORPHOLOGY
EVOLUTION
S. cerevisiae
evolutionary biology
genomics
lifespan
mitochondria
retrograde response
self-splicing intron
splicing
Publication Status: Published
Open Access location: https://elifesciences.org/articles/35330
Article Number: ARTN e35330
Appears in Collections:Clinical Sciences
Molecular Sciences
Department of Medicine



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