Activation of human Natural Killer cells by graphene oxide-templated antibody nanoclusters

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Title: Activation of human Natural Killer cells by graphene oxide-templated antibody nanoclusters
Authors: Loftus, C
Saeed, M
Davis, D
Dunlop, IE
Item Type: Journal Article
Abstract: An emerging new paradigm is that immune cell activation is controlled by transient interactions between supramolecular assemblies of receptors and ligands. Current immunotherapy biologic pharmaceuticals that activate or desensitize NK cells are, however, individual molecules that do not replicate this nanoscale organization of proteins. Here, we use nanoscale graphene oxide (NGO) as a template to generate soluble nanoscale clusters of Natural Killer cell-activating antibodies. We control nanocluster size and molecular number to mimic reported values for cell surface proteins. These NGO-templated molecular nanoclusters, used to stimulate NK cells via the CD16 receptor, successfully induced cellular activation, indicated by degranulation of cytolytic granules and IFN-γ secretion. Importantly, activation significantly exceeded that induced by the same antibodies applied as a solution of individual molecules. These results demonstrate that future immunotherapies could be enhanced by assembling immunomodulatory drugs into nanoclusters and establish NGO-templating as a candidate technology.
Issue Date: 20-Apr-2018
Date of Acceptance: 13-Apr-2018
URI: http://hdl.handle.net/10044/1/59111
DOI: https://dx.doi.org/10.1021/acs.nanolett.8b01089
ISSN: 1530-6984
Publisher: American Chemical Society
Start Page: 3282
End Page: 3289
Journal / Book Title: Nano Letters
Volume: 18
Issue: 5
Copyright Statement: © 2018 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Keywords: Bionanotechnology
NK cell
biofunctional nanoparticles
graphene oxide
immune cell activation
MD Multidisciplinary
Nanoscience & Nanotechnology
Publication Status: Published
Appears in Collections:Faculty of Engineering
Materials
Faculty of Natural Sciences



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