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Shape and Compliance of Endothelial Cells after Shear Stress In Vitro or from Different Aortic Regions: Scanning Ion Conductance Microscopy Study

Title: Shape and Compliance of Endothelial Cells after Shear Stress In Vitro or from Different Aortic Regions: Scanning Ion Conductance Microscopy Study
Authors: Potter, CMF
Schobesberger, S
Lundberg, MH
Weinberg, PD
Mitchell, JA
Gorelik, J
Item Type: Journal Article
Abstract: Objective To measure the elongation and compliance of endothelial cells subjected to different patterns of shear stress in vitro, and to compare these parameters with the elongation and compliance of endothelial cells from different regions of the intact aorta. Materials and Methods Porcine aortic endothelial cells were cultured for 6 days under static conditions or on an orbital shaker. The shaker generated a wave of medium, inducing pulsatile shear stress with a preferred orientation at the edge of the well or steadier shear stress with changing orientation at its centre. The topography and compliance of these cells and cells from the inner and outer curvature of ex vivo porcine aortic arches were measured by scanning ion conductance microscopy (SICM). Results Cells cultured under oriented shear stress were more elongated and less compliant than cells grown under static conditions or under shear stress with no preferred orientation. Cells from the outer curvature of the aorta were more elongated and less compliant than cells from the inner curvature. Conclusion The elongation and compliance of cultured endothelial cells vary according to the pattern of applied shear stress, and are inversely correlated. A similar inverse correlation occurs in the aortic arch, with variation between regions thought to experience different haemodynamic stresses.
Issue Date: 16-Feb-2012
Date of Acceptance: 4-Jan-2012
URI: http://hdl.handle.net/10044/1/58843
DOI: https://dx.doi.org/10.1371/journal.pone.0031228
ISSN: 1932-6203
Publisher: PUBLIC LIBRARY OF SCIENCE
Journal / Book Title: PLOS ONE
Volume: 7
Issue: 2
Copyright Statement: © 2012 Potter et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
British Heart Foundation
Funder's Grant Number: BB/D522711/1
NH/10/3/28574
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
LOCAL MECHANICAL-PROPERTIES
NITRIC-OXIDE RELEASE
LIVING CELLS
ATHEROGENESIS
Animals
Aorta
Cell Shape
Cells, Cultured
Endothelial Cells
Hemodynamics
Ions
Microscopy
Stress, Mechanical
Swine
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Article Number: ARTN e31228
Online Publication Date: 2012-02-16
Appears in Collections:Faculty of Engineering
Bioengineering
National Heart and Lung Institute
Faculty of Medicine



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