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Stable centrosomal roots disentangle to allow interphase centriole independence

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Title: Stable centrosomal roots disentangle to allow interphase centriole independence
Authors: Mahen, RWJ
Item Type: Journal Article
Abstract: The centrosome is a non-membrane bound cellular compartment consisting of two centrioles surrounded by a protein coat termed the pericentriolar material (PCM). Centrioles generally remain physically associated together (a phenomenon called centrosome cohesion), yet how this occurs in the absence of a bounding lipid membrane is unclear. One model posits that pericentriolar fibres formed from rootletin protein directly link centrioles, yet little is known about the structure, biophysical properties or assembly kinetics of such fibres. Here I combine live cell imaging of endogenously tagged rootletin with cell fusion, and find previously unrecognised plasticity in centrosome cohesion. Rootletin forms large, diffusionally stable, bifurcating fibres, which amass slowly on mature centrioles over many hours from anaphase. Nascent centrioles (procentrioles) in contrast do not form roots, and must be licensed to do so through polo-like kinase 1 (PLK1) activity. Transient separation of roots accompanies centriolar repositioning during the interphase, suggesting that centrioles organize as independent units, each containing discrete roots. Indeed, forced induction of duplicate centriole pairs allows independent re-shuffling of individual centrioles between the pairs. Thus collectively, these findings suggest that progressively nucleated polymers mediate the dynamic association of centrioles as either one or two interphase centrosomes, with implications for the understanding of how non-membrane bound organelles self-organise.
Issue Date: 12-Apr-2018
Date of Acceptance: 19-Mar-2018
URI: http://hdl.handle.net/10044/1/58430
DOI: https://dx.doi.org/10.1371/journal.pbio.2003998
ISSN: 1544-9173
Publisher: Public Library of Science (PLoS)
Journal / Book Title: PLoS Biology
Volume: 16
Issue: 4
Copyright Statement: © 2018 Robert Mahen. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biology
Life Sciences & Biomedicine - Other Topics
CILIARY ROOTLET
MOTHER CENTRIOLE
CELLS
CILIOGENESIS
PROTEIN
KINASE
COHESION
C-NAP1
CYCLE
ORGANIZATION
06 Biological Sciences
11 Medical And Health Sciences
07 Agricultural And Veterinary Sciences
Developmental Biology
Publication Status: Published
Article Number: e2003998
Appears in Collections:Physics
Photonics
Faculty of Natural Sciences



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