A rhamnose-inducible system for precise and temporal control of gene expression in cyanobacteria

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Title: A rhamnose-inducible system for precise and temporal control of gene expression in cyanobacteria
Author(s): Kelly, CL
Taylor, G
Hitchcock, A
Torres-Méndez, A
Heap, J
Item Type: Journal Article
Abstract: Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cyanobacteria, allowing dynamic external control of gene expression through the addition of specific inducer molecules. However, the inducible promoters used to date suffer from various drawbacks including toxicity of inducers, leaky expression in the absence of inducer and inducer photolability, the latter being particularly relevant to cyanobacteria, which, as photoautotrophs, are grown under light. Here we introduce the rhamnose-inducible rhaBAD promoter of Escherichia coli into the model freshwater cyanobacterium Synechocystis sp. PCC 6803 and demonstrate it has superior properties to previously reported cyanobacterial inducible promoter systems, such as a non-toxic, photostable, non-metabolizable inducer, a linear response to inducer concentration and crucially no basal transcription in the absence of inducer.
Publication Date: 20-Apr-2018
Date of Acceptance: 15-Mar-2018
URI: http://hdl.handle.net/10044/1/58410
DOI: https://dx.doi.org/10.1021/acssynbio.7b00435
ISSN: 2161-5063
Publisher: American Chemical Society
Start Page: 1056
End Page: 1066
Journal / Book Title: ACS Synthetic Biology
Volume: 7
Issue: 4
Copyright Statement: © 2018 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited (https://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html)
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/M011321/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
Synechocystis
cyanobacteria
rhamnose
inducible promoter
gene expression
synthetic biology
SP PCC 6803
CAMP RECEPTOR PROTEIN
ESCHERICHIA-COLI
SYNECHOCYSTIS PCC6803
SIGMA-FACTORS
TRANSCRIPTION FACTORS
ETHANOL-PRODUCTION
CIRCADIAN-RHYTHMS
RHABAD PROMOTER
BINDING-SITES
Synechocystis
cyanobacteria
gene expression
inducible promoter
rhamnose
synthetic biology
Publication Status: Published
Online Publication Date: 2018-03-15
Appears in Collections:Faculty of Natural Sciences



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