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Cell-accurate optical mapping across the entire developing heart

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Title: Cell-accurate optical mapping across the entire developing heart
Authors: Weber, M
Scherf, N
Meyer, AM
Panakova, D
Kohl, P
Huisken, J
Item Type: Journal Article
Abstract: Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca²⁺-mapping of all cells in the entire electro-mechanically uncoupled heart during the looping stage of live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs.
Issue Date: 29-Dec-2017
Date of Acceptance: 2-Dec-2017
URI: http://hdl.handle.net/10044/1/58064
DOI: https://dx.doi.org/10.7554/eLife.28307
ISSN: 2050-084X
Publisher: eLife Sciences Publications Ltd
Journal / Book Title: eLife
Volume: 6
Copyright Statement: © 2017, Weber et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited (https://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Commission of the European Communities
British Heart Foundation
Funder's Grant Number: 323099
FS/15/3/31047
Keywords: Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
CARDIAC CONDUCTION SYSTEM
LIGHT-SHEET MICROSCOPY
ZEBRAFISH HEART
MORPHOGENESIS
IDENTITY
MUTANT
Cardiac conduction
Cardiology
Light sheet microscopy
Optical mapping
Organogenesis
biophysics
developmental biology
image analysis
stem cells
structural biology
zebrafish
Publication Status: Published
Article Number: ARTN e28307
Appears in Collections:National Heart and Lung Institute
Faculty of Medicine



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