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Construction of hybrid regulated mother-specific yeast promoters for inducible differential gene expression

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Title: Construction of hybrid regulated mother-specific yeast promoters for inducible differential gene expression
Authors: Pothoulakis, G
Ellis, T
Item Type: Journal Article
Abstract: Engineered promoters with predefined regulation are a key tool for synthetic biology that enable expression on demand and provide the logic for genetic circuits. To expand the availability of synthetic biology tools for S. cerevisiae yeast, we here used hybrid promoter engineering to construct tightly-controlled, externally-inducible promoters that only express in haploid mother cells that have contributed a daughter cell to the population. This is achieved by combining elements from the native HO promoter and from a TetR-repressible synthetic promoter, with the performance of these promoters characterized by both flow cytometry and microfluidics-based fluorescence microscopy. These new engineered promoters are provided as an enabling tool for future synthetic biology applications that seek to exploit differentiation within a yeast population.
Issue Date: 22-Mar-2018
Date of Acceptance: 14-Mar-2018
URI: http://hdl.handle.net/10044/1/58046
DOI: https://dx.doi.org/10.1371/journal.pone.0194588
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Journal / Book Title: PLoS ONE
Volume: 13
Issue: 3
Copyright Statement: © 2018 Pothoulakis, Ellis. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
The Leverhulme Trust
Funder's Grant Number: BB/K006290/1
RPG-2012-562
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SACCHAROMYCES-CEREVISIAE
HO GENE
TRANSCRIPTION FACTOR
FACTOR-BINDING
MATING-TYPE
REPRESSION
POPULATIONS
PROTEINS
PLASMID
CASCADE
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Article Number: e0194588
Appears in Collections:Faculty of Engineering
Bioengineering



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