Sculpting and fusing biomimetic vesicle networks using optical tweezers

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Title: Sculpting and fusing biomimetic vesicle networks using optical tweezers
Author(s): Bolognesi, G
Friddin, MS
Salehi-Reyhani, S
Barlow, N
Brooks, NJ
Ces, O
Elani, Y
Item Type: Journal Article
Abstract: Constructing higher-order vesicle assemblies has discipline-spanning potential from responsive soft-matter materials to artificial cell networks in synthetic biology. This potential is ultimately derived from the ability to compartmentalise and order chemical species in space. To unlock such applications, spatial organisation of vesicles in relation to one another must be controlled, and techniques to deliver cargo to compartments developed. Herein, we use optical tweezers to assemble, reconfigure and dismantle networks of cell-sized vesicles that, in different experimental scenarios, we engineer to exhibit several interesting properties. Vesicles are connected through double-bilayer junctions formed via electrostatically controlled adhesion. Chemically distinct vesicles are linked across length scales, from several nanometres to hundreds of micrometres, by axon-like tethers. In the former regime, patterning membranes with proteins and nanoparticles facilitates material exchange between compartments and enables laser-triggered vesicle merging. This allows us to mix and dilute content, and to initiate protein expression by delivering biomolecular reaction components.
Publication Date: 14-May-2018
Date of Acceptance: 2-Mar-2018
URI: http://hdl.handle.net/10044/1/58029
DOI: https://dx.doi.org/10.1038/s41467-018-04282-w
ISSN: 2041-1723
Publisher: Nature Publishing Group
Journal / Book Title: Nature Communications
Volume: 9
Copyright Statement: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article ’ s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article ’ s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/N016998/1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
ARTIFICIAL CELLS
DRUG-DELIVERY
ENZYMATIC-REACTIONS
MEMBRANE NANOTUBES
GOLD NANOPARTICLES
LIPID-BILAYERS
LIPOSOMES
PROTEIN
ADHESION
SYSTEM
MD Multidisciplinary
Publication Status: Published
Article Number: 1882
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Faculty of Natural Sciences



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