Syk tyrosine kinase is required for the positive selection of immature B cells into the recirculating B cell pool

Title: Syk tyrosine kinase is required for the positive selection of immature B cells into the recirculating B cell pool
Author(s): Turner, M
Gulbranson-Judge, A
Quinn, ME
Walters, AE
MacLennan, ICM
Tybulewicz, VLJ
Item Type: Journal Article
Abstract: The tyrosine kinase Syk has been implicated as a key signal transducer from the B cell antigen receptor (BCR). We show here that mutation of the Syk gene completely blocks the maturation of immature B cells into recirculating cells and stops their entry into B cell follicles. Furthermore, using radiation chimeras we demonstrate that this developmental block is due to the absence of Syk in the B cells themselves. Syk-deficient B cells are shown to have the life span of normal immature B cells. If this is extended by over-expression of Bcl-2, they accumulate in the T zone and red pulp of the spleen in increased numbers, but still fail to mature to become recirculating follicular B cells. Despite this defect in maturation, Syk-deficient B cells were seen to give rise to switched as well as nonswitched splenic plasma cells. Normally only a proportion of immature B cells is recruited into the recirculating pool. Our results suggest that Syk transduces a BCR signal that is absolutely required for the positive selection of immature B cells into the recirculating B cell pool.
Publication Date: 15-Dec-1997
Date of Acceptance: 24-Oct-1997
URI: http://hdl.handle.net/10044/1/58000
DOI: https://dx.doi.org/10.1084/jem.186.12.2013
ISSN: 0022-1007
Publisher: Rockefeller University Press
Start Page: 2013
End Page: 2021
Journal / Book Title: Journal of Experimental Medicine
Volume: 186
Issue: 12
Copyright Statement: © 1997 Rockefeller University Press.
Keywords: Science & Technology
Life Sciences & Biomedicine
Immunology
Medicine, Research & Experimental
Research & Experimental Medicine
HEAT-STABLE ANTIGEN(HI)
MOUSE BONE-MARROW
LYMPHOID ORGANS
TRANSGENIC MICE
T-CELLS
LYMPHOCYTES
MATURATION
RECEPTOR
ACTIVATION
DIFFERENTIATION
Animals
B-Lymphocytes
Bone Marrow Cells
Cell Differentiation
Cell Separation
Enzyme Precursors
Flow Cytometry
Fluorescent Antibody Technique, Indirect
Intracellular Signaling Peptides and Proteins
Liver
Mice
Mice, Transgenic
Protein-Tyrosine Kinases
Proto-Oncogene Proteins c-bcl-2
Receptors, Antigen, B-Cell
Signal Transduction
Spleen
Syk Kinase
Liver
Spleen
B-Lymphocytes
Bone Marrow Cells
Animals
Mice, Transgenic
Mice
Enzyme Precursors
Intracellular Signaling Peptides and Proteins
Receptors, Antigen, B-Cell
Proto-Oncogene Proteins c-bcl-2
Fluorescent Antibody Technique, Indirect
Flow Cytometry
Cell Separation
Signal Transduction
Cell Differentiation
Protein-Tyrosine Kinases
Syk Kinase
Science & Technology
Life Sciences & Biomedicine
Immunology
Medicine, Research & Experimental
Research & Experimental Medicine
HEAT-STABLE ANTIGEN(HI)
MOUSE BONE-MARROW
LYMPHOID ORGANS
TRANSGENIC MICE
T-CELLS
LYMPHOCYTES
MATURATION
RECEPTOR
ACTIVATION
DIFFERENTIATION
11 Medical And Health Sciences
Immunology
Publication Status: Published
Appears in Collections:Department of Medicine
Faculty of Medicine



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