Optimising the collection of female genital tract fluid for cytokine analysis in pregnant women

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Title: Optimising the collection of female genital tract fluid for cytokine analysis in pregnant women
Authors: Short, CS
Quinlan, R
Bennett, P
Shattock, R
Taylor, G
Item Type: Journal Article
Abstract: Introduction: To better understand the immunology of pregnancy, study of female genital tract fluid (FGF) is desirable. However the optimum method of collection of FGF in pregnant women for immunological methods, specifically cytokine measurement, is unknown. Methods: A prospective study of HIV-uninfected pregnant women comparing two methods of FGF collection: polyvinyl acetal sponge collection of cervical fluid (CF) and menstrual cup collection of cervicovaginal fluid (CVF). Samples were collected at 3 time points across the second and third trimesters: 14-21, 22-25 and 26-31 weeks. Multiplex chemi-luminescent assays were used to measure: IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13 and TNF-α. Optimal methodology for cytokine normalisation (sample weight, volume and total protein) was explored. Results All cytokines were measurable in both fluid types. IL-1β, IL-8 and IL-6 were detected at the highest concentrations (ranking order CF > CVF > plasma). CVF collection was simpler, provided the largest volume of sample (median 0.5g) with the potential for undiluted usage, and allowed for self-insertion. CF cytokine concentrations were intrinsically associated with sample weight and protein concentration however CVF cytokines were independent of these. Conclusion: Both methods of collection are robust for measurement of FGF cytokines during pregnancy. We recommend CVF collection using a menstrual cup as a viable option in pregnant women for high dimensional biological techniques.
Issue Date: 1-Jul-2018
Date of Acceptance: 15-Feb-2018
URI: http://hdl.handle.net/10044/1/57927
DOI: https://dx.doi.org/10.1016/j.jim.2018.03.014
ISSN: 0022-1759
Publisher: Elsevier
Start Page: 15
End Page: 20
Journal / Book Title: Journal of Immunological Methods
Volume: 458
Copyright Statement: © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/)
Sponsor/Funder: Wellcome Trust
Wellcome trust
Funder's Grant Number: 102757/Z/13/Z
WT102757/Z/13/Z
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Immunology
Biochemistry & Molecular Biology
Female genital tract fluid
Cervicovaginal fluid
Pregnancy
Cytokines
Menstrual cup
Ophthalmic sponge
SPONTANEOUS PRETERM BIRTH
CERVICAL-VAGINAL FLUID
CERVICOVAGINAL SECRETIONS
INFLAMMATORY CYTOKINES
BACTERIAL VAGINOSIS
NONPREGNANT WOMEN
IMMUNE MARKERS
IMMUNOGLOBULINS
MUCUS
INTERLEUKIN-1-BETA
1107 Immunology
1113 Ophthalmology And Optometry
Publication Status: Published
Online Publication Date: 2018-04-03
Appears in Collections:Division of Surgery
Department of Medicine
Faculty of Medicine



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