A Computed Microtomography Method for Understanding Epiphyseal Growth Plate Fusion

File Description SizeFormat 
fmats-04-00048.pdfPublished version1.78 MBAdobe PDFDownload
Title: A Computed Microtomography Method for Understanding Epiphyseal Growth Plate Fusion
Author(s): Staines, KA
Madi, K
Javaheri, B
Lee, PD
Pitsillides, AA
Item Type: Journal Article
Abstract: The epiphyseal growth plate is a developmental region responsible for linear bone growth, in which chondrocytes undertake a tightly regulated series of biological pro- cesses. Concomitant with the cessation of growth and sexual maturation, the human growth plate undergoes progressive narrowing, and ultimately disappears. Despite the crucial role of this growth plate fusion “bridging” event, the precise mechanisms by which it is governed are complex and yet to be established. Progress is hindered by the current methods for growth plate visualization; these are invasive and largely rely on histological procedures. Here, we describe our non-invasive method utilizing synchro- tron X-ray computed microtomography for the examination of growth plate bridging, which ultimately leads to its closure coincident with termination of further longitudinal bone growth. We then apply this method to a dataset obtained from a benchtop micro computed tomography scanner to highlight its potential for wide usage. Furthermore, we conduct finite element modeling at the micron-scale to reveal the effects of growth plate bridging on local tissue mechanics. Employment of these 3D analyses of growth plate bone bridging is likely to advance our understanding of the physiological mechanisms that control growth plate fusion.
Publication Date: 23-Jan-2018
Date of Acceptance: 22-Dec-2017
URI: http://hdl.handle.net/10044/1/57511
DOI: https://dx.doi.org/10.3389/fmats.2017.00048
ISSN: 2296-8016
Publisher: FRONTIERS MEDIA SA
Journal / Book Title: FRONTIERS IN MATERIALS
Volume: 4
Copyright Statement: © 2018 Staines, Madi, Javaheri, Lee and Pitsillides. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Keywords: Science & Technology
Technology
Materials Science, Multidisciplinary
Materials Science
growth plate
mouse
bone
synchrotron
computed tomography
fusion
BONE BRIDGE FORMATION
ENDOCHONDRAL OSSIFICATION
SKELETAL MINERALIZATION
TISSUE DIFFERENTIATION
CARTILAGE
CHONDROCYTE
ESTROGEN
MODEL
COMPRESSION
OSTEOARTHRITIS
bone
computed tomography
fusion
growth plate
mouse
synchrotron
Science & Technology
Technology
Materials Science, Multidisciplinary
Materials Science
growth plate
mouse
bone
synchrotron
computed tomography
fusion
BONE BRIDGE FORMATION
ENDOCHONDRAL OSSIFICATION
SKELETAL MINERALIZATION
TISSUE DIFFERENTIATION
CARTILAGE
CHONDROCYTE
ESTROGEN
MODEL
COMPRESSION
OSTEOARTHRITIS
Publication Status: Published
Article Number: UNSP 48
Open Access location: https://doi.org/10.3389/fmats.2017.00048
Appears in Collections:Materials



Items in Spiral are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons