Gut microbiota interacts with markers of adipose tissue Browning, insulin action and plasma acetate in morbid obesity

File Description SizeFormat 
last submitted version.pdfAccepted version784.61 kBAdobe PDFView/Open
Title: Gut microbiota interacts with markers of adipose tissue Browning, insulin action and plasma acetate in morbid obesity
Authors: Moreno-Navarrete, JM
Serino, M
Blasco-Baque, V
Azalbert, V
Barton, RH
Cardellini, M
Latorre, J
Ortega, F
Sabater-Masdeu, M
Burcelin, R
Dumas, M-E
Ricart, W
Federici, M
Fernández-Real, JM
Item Type: Journal Article
Abstract: SCOPE: To examine the potential relationship among gene expression markers of adipose tissue browning, gut microbiota, and insulin sensitivity in humans. METHODS AND RESULTS: Gut microbiota composition and gene markers of browning are analyzed in subcutaneous (SAT) and visceral (VAT) adipose tissue from morbidly obese subjects (n = 34). Plasma acetate is measured through 1 H NMR and insulin sensitivity using euglycemic hyperinsulinemic clamp. Subjects with insulin resistance show an increase in the relative abundance (RA) of the phyla Bacteroidetes and Proteobacteria while RA of Firmicutes is decreased. In all subjects, Firmicutes RA is negatively correlated with HbA1c and fasting triglycerides, whereas Proteobacteria RA was negatively correlated with insulin sensitivity. Firmicutes RA is positively associated with markers of brown adipocytes (PRDM16, UCP1, and DIO2) in SAT, but not in VAT. Multivariate regression analysis indicates that Firmicutes RA contributes significantly to SAT PRDM16, UCP1, and DIO2 mRNA variance after controlling for age, BMI, HbA1c , or insulin sensitivity. Interestingly, Firmicutes RA, specifically those bacteria belonging to the Ruminococcaceae family, is positively associated with plasma acetate levels, which are also linked to SAT PRDM16 mRNA and insulin sensitivity. CONCLUSION: Gut microbiota composition is linked to adipose tissue browning and insulin action in morbidly obese subjects, possibly through circulating acetate.
Issue Date: 29-Dec-2017
Date of Acceptance: 3-Nov-2017
ISSN: 1613-4125
Publisher: Wiley
Journal / Book Title: Molecular Nutrition and Food Research
Volume: 62
Issue: 3
Copyright Statement: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the pre-peer reviewed version of the following article, which has been published in final form at
Sponsor/Funder: Commission of the European Communities
Funder's Grant Number: 241913
Keywords: Science & Technology
Life Sciences & Biomedicine
Food Science & Technology
adipose tissue
insulin sensitivity
0908 Food Sciences
1111 Nutrition And Dietetics
Nutrition & Dietetics
Food Science
Publication Status: Published
Conference Place: Germany
Article Number: 1700721
Appears in Collections:Division of Surgery
Faculty of Medicine

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons