Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse

Title: Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse
Authors: Tsipouri, V
Curtin, JA
Nolan, PM
Vizor, L
Parsons, CA
Clapham, CM
Latham, ID
Rooke, LJ
Martin, JE
Peters, J
Hunter, AJ
Rogers, D
Rastan, S
Brown, SDM
Fisher, EMC
Spurr, NK
Gray, IC
Item Type: Journal Article
Abstract: Three mutant mice with pigmentation phenotypes were recovered from a genomewide random mouse chemical mutagenesis study. White toes (Whto; MGI:1861986), Belly spot and white toes (Bswt; MGI:2152776) and Dark footpads 2 (Dfp2; MGI:1861991) were identified following visual inspection of progeny from a male exposed to the point mutagen ethylnitrosourea (ENU). In order to rapidly localize the causative mutations, genome-wide linkage scans were performed on pooled DNA samples from backcross animals for each mutant line. Whto was mapped to proximal mouse chromosome (Mmu) 7 between Cen (the centromere) and D7Mit112 (8.0 cM from the centromere), Bswt was mapped to centric Mmul between D1Mit214 (32.1 cM) and D1Mit480 (32.8 cM) and Dfp2 was mapped to proximalMmu4 between Cen and D4Mit18 (5.2 cM). Whto, Bswt and Dfp2 may provide novel starting points in furthering the elucidation of genetic and biochemical pathways relevant to pigmentation and associated biological processes.
Issue Date: 1-Mar-2004
Date of Acceptance: 19-Dec-2003
URI: http://hdl.handle.net/10044/1/57301
DOI: https://dx.doi.org/10.1002/cfg.382
ISSN: 1531-6912
Publisher: Hindawi Publishing Corporation
Start Page: 123
End Page: 127
Journal / Book Title: Comparative and Functional Genomics
Volume: 5
Issue: 2
Copyright Statement: © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (https://creativecommons.org/licenses/by/3.0/).
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Genetics & Heredity
mouse
pigmentation mutant
ethylnitrosourea
mutagenesis
linkage
GENE-FUNCTION
MICE
MIGRATION
MUTATION
DISEASE
Microbiology
Publication Status: Published
Appears in Collections:Division of Surgery
Division of Cancer
Faculty of Medicine



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