Influence of cholecystokinin-8 on compound nerve action potentials from ventral gastric vagus in rats

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Title: Influence of cholecystokinin-8 on compound nerve action potentials from ventral gastric vagus in rats
Authors: Mirza, K
Alenda, A
Eftekhar, A
Grossman, N
Nikolic, K
Bloom, S
Toumazou, C
Item Type: Journal Article
Abstract: Objective: Vagus Nerve Stimulation (VNS) has shown great promise as a potential therapy for a number of conditions, such as epilepsy, depression and for Neurometabolic Therapies , especially for treating obesity. The objective of this study was to characterise the left ventral subdiaphragmatic gastric trunk of vagus nerve (SubDiaGVN) and to analyse the influence of intravenous injection of gut hormone cholecystokinin octapeptide (CCK-8) on compound nerve action potential (CNAP) observed on the same branch, with the aim of understanding the impact of hormones on VNS and incorporating the methods and results into closed loop implant design. Methods: The cervical region of the left vagus nerve (CerVN) of male Wistar rats was stimulated with electric current and the elicited CNAPs were recorded on the SubDiaGVN under four different conditions: Control (no injection), Saline , CCK1 (100 pmol/kg) and CCK2 (1000 pmol/kg) injections. Results: We identified the presence of A δ , B, C1, C2, C3 and C4 fibres with their respective velocity ranges. Intravenous administration of CCK in vivo results in selective, statistically significant reduction of CNAP components originating from A and B fibres, but with no discernible effect on the C fibres in n=7 animals. The affected CNAP components exhibit statistically significant ( p Saline − CCK1 = 0 . 02 and p Saline − CCK2 = 0 . 007) higher normalized stimulation thresholds. Conclusion: This approach of characterising the vagus nerve can be used in closed loop systems to determine when to initiate VNS and also to tune the stimulation dose, which is patient specific and changes over time.
Issue Date: 9-Apr-2018
Date of Acceptance: 4-Feb-2018
ISSN: 0129-0657
Publisher: World Scientific Publishing
Journal / Book Title: International Journal of Neural Systems
Volume: 28
Issue: 9
Copyright Statement: © 2018 The Author(s). This is an Open Access article published by World Scientific Publishing Company. It is distributed under the terms of the Creative Commons Attribution 4.0 (CC-BY) License. Further distribution of this work is permitted, provided the original work is properly cited.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/K009842/1
Keywords: Science & Technology
Computer Science, Artificial Intelligence
Computer Science
Compound nerve action potentials
subdiaphragmatic vagus nerve
vagus nerve stimulation
closed loop system
0801 Artificial Intelligence And Image Processing
1702 Cognitive Science
Artificial Intelligence & Image Processing
Publication Status: Published
Article Number: 1850006
Appears in Collections:Faculty of Engineering
Electrical and Electronic Engineering
Department of Medicine
Faculty of Medicine

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