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Cell type of origin dictates the route to pluripotency

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Nefzger et al Cell Reports 2017.pdfPublished version11.05 MBAdobe PDFView/Open
Title: Cell type of origin dictates the route to pluripotency
Authors: Nefzger, CM
Rossello, FJ
Chen, J
Liu, X
Knaupp, AS
Firas, J
Paynter, JM
Pflueger, J
Buckberry, S
Lim, SM
Williams, B
Alaei, S
Faye-Chauhan, K
Petretto, E
Nilsson, SK
Lister, R
Ramialison, M
Powell, DR
Rackham, OJL
Polo, JM
Item Type: Journal Article
Abstract: Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblasts as a universal model for the study of the reprogramming process and provide crucial insights about iPSC generation from alternative cell sources.
Issue Date: 5-Dec-2017
Date of Acceptance: 8-Nov-2017
URI: http://hdl.handle.net/10044/1/57237
DOI: https://dx.doi.org/10.1016/j.celrep.2017.11.029
ISSN: 2211-1247
Publisher: Elsevier
Start Page: 2649
End Page: 2660
Journal / Book Title: Cell Reports
Volume: 21
Issue: 10
Copyright Statement: © 2017 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
HEMATOPOIETIC STEM-CELLS
MASS CYTOMETRY
MOLECULAR ROADMAP
SOMATIC-CELLS
IPS CELLS
EXPRESSION
MARKERS
MOUSE
Egr1
fibroblasts
induced pluripotent stem cells
keratinocytes
neutrophils
reprogramming
transcriptional dynamics
Publication Status: Published
Appears in Collections:Clinical Sciences
Molecular Sciences
Faculty of Medicine



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