Cell geometry dependent changes in plasma membrane order direct stem cell signalling and fate

File Description SizeFormat 
2017-vErlach-NatMat-accepted.pdfAccepted version1.68 MBAdobe PDFView/Open
Title: Cell geometry dependent changes in plasma membrane order direct stem cell signalling and fate
Authors: Von Erlach, T
Bertazzo, S
Wozniak, MA
Horejs, C
Maynard, SA
Attwood, S
Robinson, BK
Autefage, H
Kallepitis, C
Del Rio Hernandez, A
Chen, CS
Goldoni, S
Stevens, MM
Item Type: Journal Article
Abstract: Cell size and shape affect cellular processes such as cell survival, growth and differentiation1,2,3,4, thus establishing cell geometry as a fundamental regulator of cell physiology. The contributions of the cytoskeleton, specifically actomyosin tension, to these effects have been described, but the exact biophysical mechanisms that translate changes in cell geometry to changes in cell behaviour remain mostly unresolved. Using a variety of innovative materials techniques, we demonstrate that the nanostructure and lipid assembly within the cell plasma membrane are regulated by cell geometry in a ligand-independent manner. These biophysical changes trigger signalling events involving the serine/threonine kinase Akt/protein kinase B (PKB) that direct cell-geometry-dependent mesenchymal stem cell differentiation. Our study defines a central regulatory role by plasma membrane ordered lipid raft microdomains in modulating stem cell differentiation with potential translational applications.
Issue Date: 12-Feb-2018
Date of Acceptance: 18-Dec-2017
URI: http://hdl.handle.net/10044/1/57125
DOI: https://dx.doi.org/10.1038/s41563-017-0014-0
ISSN: 1476-1122
Publisher: Nature Publishing Group
Start Page: 237
End Page: 242
Journal / Book Title: Nature Materials
Volume: 17
Copyright Statement: © 2018 Macmillan Publishers limited, part of Springer Nature. All rights reserve
Sponsor/Funder: Commission of the European Communities
Commission of the European Communities
Wellcome Trust
Funder's Grant Number: ERC-2007-StG-206807
282051
098411/Z/12/Z
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Physical
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Materials Science
Physics
LIPID RAFTS
CAVEOLAE
MICROTUBULES
MICRODOMAINS
GROWTH
SWITCH
MD Multidisciplinary
Nanoscience & Nanotechnology
Publication Status: Published
Appears in Collections:Faculty of Engineering
Materials
Bioengineering
Faculty of Natural Sciences



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commonsx