Light-triggered enzymatic reactions in nested vesicle reactors

File Description SizeFormat 
s41467-018-03491-7.pdfPublished version844.31 kBAdobe PDFDownload
Title: Light-triggered enzymatic reactions in nested vesicle reactors
Author(s): Hindley, JW
Elani, Y
McGilvery, CM
Ali, S
Bevan, CL
Law, R
Ces, O
Item Type: Journal Article
Abstract: Cell-sized vesicles have tremendous potential both as miniaturised pL reaction vessels and in bottom-up synthetic biology as chassis for artificial cells. In both these areas the introduction of light-responsive modules affords increased functionality, for example, to initiate enzymatic reactions in the vesicle interior with spatiotemporal control. Here we report a system composed of nested vesicles where the inner compartments act as phototransducers, responding to ultraviolet irradiation through diacetylene polymerisation-induced pore formation to initiate enzymatic reactions. The controlled release and hydrolysis of a fluorogenic β-galactosidase substrate in the external compartment is demonstrated, where the rate of reaction can be modulated by varying ultraviolet exposure time. Such cell-like nested microreactor structures could be utilised in fields from biocatalysis through to drug delivery.
Publication Date: 15-Mar-2018
Date of Acceptance: 29-Jan-2018
URI: http://hdl.handle.net/10044/1/56705
DOI: https://dx.doi.org/10.1038/s41467-018-03491-7
ISSN: 2041-1723
Publisher: Nature Publishing Group
Journal / Book Title: Nature Communications
Volume: 9
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/J017566/1
EP/N016998/1
Copyright Statement: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article ’ s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article ’ s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/
Keywords: MD Multidisciplinary
Publication Status: Published
Article Number: 1093
Appears in Collections:Faculty of Engineering
Materials
Division of Surgery
Chemistry
Biological and Biophysical Chemistry
Division of Cancer
Faculty of Medicine
Faculty of Natural Sciences



Items in Spiral are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons