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Overloaded and stressed: whole-cell considerations for bacterial synthetic biology.

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Title: Overloaded and stressed: whole-cell considerations for bacterial synthetic biology.
Authors: Borkowski, O
Ceroni, F
Stan, GB
Ellis, T
Item Type: Journal Article
Abstract: The predictability and robustness of engineered bacteria depend on the many interactions between synthetic constructs and their host cells. Expression from synthetic constructs is an unnatural load for the host that typically reduces growth, triggers stresses and leads to decrease in performance or failure of engineered cells. Work in systems and synthetic biology has now begun to address this through new tools, methods and strategies that characterise and exploit host-construct interactions in bacteria. Focusing on work in E. coli, we review here a selection of the recent developments in this area, highlighting the emerging issues and describing the new solutions that are now making the synthetic biology community consider the cell just as much as they consider the construct.
Issue Date: 2-Aug-2016
Date of Acceptance: 2-Aug-2016
URI: http://hdl.handle.net/10044/1/54638
DOI: https://dx.doi.org/10.1016/j.mib.2016.07.009
ISSN: 1879-0364
Publisher: Elsevier
Start Page: 123
End Page: 130
Journal / Book Title: Current Opinion in Microbiology
Volume: 33
Copyright Statement: © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/J021849/1
EP/K020617/1
EP/M002306/1
EP/M002187/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
ESCHERICHIA-COLI
GENE-EXPRESSION
GROWTH-RATE
CIRCUIT PERFORMANCE
DESIGN
IMPLEMENTATION
DEGRADATION
PROTEINS
DYNAMICS
SYSTEMS
Escherichia coli
DNA, Bacterial
Genetic Engineering
Models, Biological
Synthetic Biology
0605 Microbiology
1108 Medical Microbiology
Publication Status: Published
Appears in Collections:Bioengineering
Chemical Engineering



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