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Synthetic gene regulation for independent external induction of the Saccharomyces cerevisiae pseudohyphal growth phenotype

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Title: Synthetic gene regulation for independent external induction of the Saccharomyces cerevisiae pseudohyphal growth phenotype
Authors: Pothoulakis, G
Ellis, T
Item Type: Journal Article
Abstract: Pseudohyphal growth is a multicellular phenotype naturally performed by wild budding yeast cells in response to stress. Unicellular yeast cells undergo gross changes in their gene regulation and elongate to form branched filament structures consisting of connected cells. Here, we construct synthetic gene regulation systems to enable external induction of pseudohyphal growth in Saccharomyces cerevisiae. By controlling the expression of the natural PHD1 and FLO8 genes we are able to trigger pseudohyphal growth in both diploid and haploid yeast, even in different types of rich media. Using this system, we also investigate how members of the BUD gene family control filamentation in haploid cells. Finally, we employ a synthetic genetic timer network to control pseudohyphal growth and further explore the reversibility of differentiation. Our work demonstrates that synthetic regulation can exert control over a complex multigene phenotype and offers opportunities for rationally modifying the resulting multicellular structure.
Issue Date: 22-Jan-2018
Date of Acceptance: 4-Dec-2017
URI: http://hdl.handle.net/10044/1/54484
DOI: https://dx.doi.org/10.1038/s42003-017-0008-0
ISSN: 2399-3642
Publisher: Nature Publishing Group
Journal / Book Title: Communications Biology
Volume: 1
Copyright Statement: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. © The Author(s) 2018
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
The Leverhulme Trust
Funder's Grant Number: BB/K006290/1
RPG-2012-562
Publication Status: Published
Article Number: 7
Appears in Collections:Faculty of Engineering
Bioengineering



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