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Rheological Droplet Interface Bilayers (rheo-DIBs): Probing the Unstirred Water Layer Effect on Membrane Permeability via Spinning Disk Induced Shear Stress

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Title: Rheological Droplet Interface Bilayers (rheo-DIBs): Probing the Unstirred Water Layer Effect on Membrane Permeability via Spinning Disk Induced Shear Stress
Authors: Barlow, NE
Bolognesi, G
Haylock, S
Flemming, AJ
Brooks, NJ
Barter, LMC
Ces, O
Item Type: Journal Article
Abstract: A new rheological droplet interface bilayer (rheo-DIB) device is presented as a tool to apply shear stress on biological lipid membranes. Despite their exciting potential for affecting high-throughput membrane translocation studies, permeability assays conducted using DIBs have neglected the effect of the unstirred water layer (UWL). However as demonstrated in this study, neglecting this phenomenon can cause significant underestimates in membrane permeability measurements which in turn limits their ability to predict key processes such as drug translocation rates across lipid membranes. With the use of the rheo-DIB chip, the effective bilayer permeability can be modulated by applying shear stress to the droplet interfaces, inducing flow parallel to the DIB membranes. By analysing the relation between the effective membrane permeability and the applied stress, both the intrinsic membrane permeability and UWL thickness can be determined for the first time using this model membrane approach, thereby unlocking the potential of DIBs for undertaking diffusion assays. The results are also validated with numerical simulations.
Issue Date: 14-Dec-2017
Date of Acceptance: 1-Dec-2017
URI: http://hdl.handle.net/10044/1/54428
DOI: https://dx.doi.org/10.1038/s41598-017-17883-0
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 7
Copyright Statement: permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (E
Commission of the European Communities
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Engineering and Physical Sciences Research Council
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: EP/J017566/1
EP/I037180/1
607466
EP/K503733/1
EP/K503381/1
EP/L015498/1
BB/SCA/Imperial/17
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MICROFLUIDIC DEVICE
TRANSPORT KINETICS
PLANT-CELLS
FROG-SKIN
ASSAY
CHOLESTEROL
DIFFUSION
THICKNESS
ABSORPTION
INTESTINE
Publication Status: Published
Article Number: 17551
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Faculty of Natural Sciences



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