In silico analysis of the small molecule content of outer membrane vesicles produced by Bacteroides thetaiotaomicron indicates an extensive metabolic link between microbe and host

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Title: In silico analysis of the small molecule content of outer membrane vesicles produced by Bacteroides thetaiotaomicron indicates an extensive metabolic link between microbe and host
Author(s): Bryant, WA
Stentz, R
Le Gall, G
Sternberg, MJE
Carding, SR
Wilhelm, T
Item Type: Journal Article
Abstract: The interactions between the gut microbiota and its host are of central importance to the health of the host. Outer membrane vesicles (OMVs) are produced ubiquitously by Gram-negative bacteria including the gut commensal Bacteroides thetaiotaomicron. These vesicles can interact with the host in various ways but until now their complement of small molecules has not been investigated in this context. Using an untargeted high-coverage metabolomic approach we have measured the small molecule content of these vesicles in contrasting in vitro conditions to establish what role these metabolites could perform when packed into these vesicles. B. thetaiotaomicron packs OMVs with a highly conserved core set of small molecules which are strikingly enriched with mouse-digestible metabolites and with metabolites previously shown to be associated with colonization of the murine GIT. By use of an expanded genome-scale metabolic model of B. thetaiotaomicron and a potential host (the mouse) we have established many possible metabolic pathways between the two organisms that were previously unknown, and have found several putative novel metabolic functions for mouse that are supported by gene annotations, but that do not currently appear in existing mouse metabolic networks. The lipidome of these OMVs bears no relation to the mouse lipidome, so the purpose of this particular composition of lipids remains unclear. We conclude from this analysis that through intimate symbiotic evolution OMVs produced by B. thetaiotaomicron are likely to have been adopted as a conduit for small molecules bound for the mammalian host in vivo.
Publication Date: 8-Dec-2017
Date of Acceptance: 24-Nov-2017
URI: http://hdl.handle.net/10044/1/54386
DOI: https://dx.doi.org/10.3389/fmicb.2017.02440
ISSN: 1664-302X
Publisher: Frontiers Media
Journal / Book Title: Frontiers in Microbiology
Volume: 8
Copyright Statement: © 2017 Bryant, Stentz, Le Gall, Sternberg, Carding and Wilhelm. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Sponsor/Funder: Biotechnology and Biological Sciences Research Cou
Biotechnology and Biological Sciences Research Council
Funder's Grant Number: PR5858-000-A
PR5858-000-A
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
Bacteroides thetaiotaomicron VPI-5482
outer membrane vesicle
metabolomics
genome-scale metabolic modeling
host-microbe interaction
GRAM-NEGATIVE BACTERIA
HUMAN GUT
IMMUNE
BIOGENESIS
OPTIMIZATION
COMMENSAL
RESPONSES
NETWORKS
SYMBIONT
DISEASE
Bacteroides thetaiotaomicron VPI-5482
genome-scale metabolic modeling
host–microbe interaction
metabolomics
outer membrane vesicle
Publication Status: Published
Article Number: 2440
Appears in Collections:Faculty of Natural Sciences



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