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Evolution of the Staphylococcus argenteus ST2250 Clone in Northeastern Thailand is linked with the acquisition of livestock-associated Staphylococcal genes

Title: Evolution of the Staphylococcus argenteus ST2250 Clone in Northeastern Thailand is linked with the acquisition of livestock-associated Staphylococcal genes
Authors: Moradigaravand, D
Jamrozy, D
Mostowy, R
Anderson, A
Nickerson, EK
Thaipadungpanit, J
Wuthiekanun, V
Limmathurotsakul, D
Tandhavanant, S
Wikraiphat, C
Wongsuvan, G
Teerawattanasook, N
Jutrakul, Y
Srisurat, N
Chaimanee, P
Eoin West, T
Blane, B
Parkhill, J
Chantratita, N
Peacock, SJ
Item Type: Journal Article
Abstract: Staphylococcus argenteus is a newly named species previously described as a divergent lineage of Staphylococcus aureus that has recently been shown to have a global distribution. Despite growing evidence of the clinical importance of this species, knowledge about its population epidemiology and genomic architecture is limited. We used whole-genome sequencing to evaluate and compare S. aureus (n = 251) and S. argenteus (n = 68) isolates from adults with staphylococcal sepsis at several hospitals in northeastern Thailand between 2006 and 2013. The majority (82%) of the S. argenteus isolates were of multilocus sequence type 2250 (ST2250). S. aureus was more diverse, although 43% of the isolates belonged to ST121. Bayesian analysis suggested an S. argenteus ST2250 substitution rate of 4.66 (95% confidence interval [CI], 3.12 to 6.38) mutations per genome per year, which was comparable to the S. aureus ST121 substitution rate of 4.07 (95% CI, 2.61 to 5.55). S. argenteus ST2250 emerged in Thailand an estimated 15 years ago, which contrasts with the S. aureus ST1, ST88, and ST121 clades that emerged around 100 to 150 years ago. Comparison of S. argenteus ST2250 genomes from Thailand and a global collection indicated a single introduction into Thailand, followed by transmission to local and more distant countries in Southeast Asia and further afield. S. argenteus and S. aureus shared around half of their core gene repertoire, indicating a high level of divergence and providing strong support for their classification as separate species. Several gene clusters were present in ST2250 isolates but absent from the other S. argenteus and S. aureus study isolates. These included multiple exotoxins and antibiotic resistance genes that have been linked previously with livestock-associated S. aureus, consistent with a livestock reservoir for S. argenteus These genes appeared to be associated with plasmids and mobile genetic elements and may have contributed to the biological success of ST2250.IMPORTANCE In this study, we used whole-genome sequencing to understand the genome evolution and population structure of a systematic collection of ST2250 S. argenteus isolates. A newly identified ancestral species of S. aureus, S. argenteus has become increasingly known as a clinically important species that has been reported recently across various countries. Our results indicate that S. argenteus has spread at a relatively rapid pace over the past 2 decades across northeastern Thailand and acquired multiple exotoxin and antibiotic resistance genes that have been linked previously with livestock-associated S. aureus Our findings highlight the clinical importance and potential pathogenicity of S. argenteus as a recently emerging pathogen.
Issue Date: 5-Jul-2017
Date of Acceptance: 8-Jun-2017
URI: http://hdl.handle.net/10044/1/54365
DOI: https://dx.doi.org/10.1128/mBio.00802-17
ISSN: 2150-7511
Publisher: American Society for Microbiology
Journal / Book Title: mBio
Volume: 8
Issue: 4
Copyright Statement: © 2017 Moradigaravand et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/)
Keywords: Staphylococcus argenteus
Staphylococcus aureus
antibiotic resistance
genomic epidemiology
0605 Microbiology
Publication Status: Published
Conference Place: United States
Appears in Collections:Faculty of Medicine
Epidemiology, Public Health and Primary Care



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