A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.

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Title: A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.
Author(s): Wen, KY
Cameron, L
Chappell, J
Jensen, K
Bell, DJ
Kelwick, R
Kopniczky, M
Davies, JC
Filloux, A
Freemont, PS
Item Type: Journal Article
Abstract: Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC-MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.
Publication Date: 5-Oct-2017
Date of Acceptance: 13-Sep-2017
URI: http://hdl.handle.net/10044/1/53616
DOI: https://dx.doi.org/10.1021/acssynbio.7b00219
ISSN: 2161-5063
Publisher: American Chemical Society
Start Page: 2293
End Page: 2301
Journal / Book Title: ACS Synthetic Biology
Volume: 6
Issue: 12
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/N023854/1
Copyright Statement: This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
Pseudomonas aeruginosa
cystic fibrosis
quorum sensing
cell-free synthetic biology
biosensor
CYSTIC-FIBROSIS PATIENTS
N-ACYLHOMOSERINE LACTONES
L-HOMOSERINE LACTONE
PSEUDOMONAS-AERUGINOSA
SIGNALING MOLECULES
BACTERIAL BIOFILM
SYNTHETIC BIOLOGY
MASS-SPECTROMETRY
VIRULENCE GENES
EXPRESSION
Pseudomonas aeruginosa
biosensor
cell-free synthetic biology
cystic fibrosis
quorum sensing
Pseudomonas aeruginosa
biosensor
cell-free synthetic biology
cystic fibrosis
quorum sensing
Publication Status: Published
Appears in Collections:National Heart and Lung Institute
Department of Medicine
Faculty of Medicine
Faculty of Natural Sciences



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