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Raman spectroscopy imaging reveals interplay between atherosclerosis and medial calcification in human aorta

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Title: Raman spectroscopy imaging reveals interplay between atherosclerosis and medial calcification in human aorta
Authors: You, AYF
Bergholt, MS
St-Pierre, JP
Chester, AH
Yacoub, MH
Bertazzo, S
Stevens, MM
Item Type: Journal Article
Abstract: Medial calcification in the human aorta accumulates during aging and is known to be aggravated in several diseases. Atherosclerosis, another major cause of cardiovascular calcification, shares some common aggravators. However, the mechanisms of cardiovascular calcification remain poorly understood. To elucidate the relationship between medial aortic calcification and atherosclerosis, we characterized the cross-sectional distributions of the predominant minerals in aortic tissue, apatite and whitlockite, and the associated extracellular matrix. We also compared the cellular changes between atherosclerotic and nonatherosclerotic human aortic tissues. This was achieved through the development of Raman spectroscopy imaging methods that adapted algorithms to distinguish between the major biomolecules present within these tissues. We present a relationship between apatite, cholesterol, and triglyceride in atherosclerosis, with the relative amount of all molecules concurrently increased in the atherosclerotic plaque. Further, the increase in apatite was disproportionately large in relation to whitlockite in the aortic media directly underlying a plaque, indicating that apatite is more pathologically significant in atherosclerosis-aggravated medial calcification. We also discovered a reduction of β-carotene in the whole aortic intima, including a plaque in atherosclerotic aortic tissues compared to nonatherosclerotic tissues. This unprecedented biomolecular characterization of the aortic tissue furthers our understanding of pathological and physiological cardiovascular calcification events in humans.
Issue Date: 6-Dec-2017
Date of Acceptance: 9-Nov-2017
URI: http://hdl.handle.net/10044/1/53415
DOI: https://dx.doi.org/10.1126/sciadv.1701156
ISSN: 2375-2548
Publisher: American Association for the Advancement of Science
Journal / Book Title: Science Advances
Volume: 3
Issue: 12
Copyright Statement: Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
Sponsor/Funder: Wellcome Trust
Medical Research Council (MRC)
Medical Research Council (MRC)
Medical Research Council (MRC)
Wellcome Trust
Commission of the European Communities
Commission of the European Communities
Funder's Grant Number: 088844/Z/09/Z
MR/K026666/1
MR/L012677/1
MR/K026682/1
097816/Z/11/B
ERC-2013-CoG-616417
701713
Publication Status: Published
Article Number: e1701156
Appears in Collections:Faculty of Engineering
Materials
National Heart and Lung Institute
Faculty of Medicine
Faculty of Natural Sciences



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