Altmetric

Bone marrow manifestations in multicentric Castleman disease

File Description SizeFormat 
Bone+marrow+findings+in+MCD+V3aBOWER.docAccepted version189.5 kBMicrosoft WordView/Open
Title: Bone marrow manifestations in multicentric Castleman disease
Authors: Ibrahim, HAH
Balachandran, K
Bower, M
Naresh, KN
Item Type: Journal Article
Abstract: This study aimed to document the morphological and immunophenotypic features, and describe the diagnostic features of bone marrow (BM) involvement in human herpes virus 8 Multicentric Castleman disease (HHV8-MCD). BM trephine biopsy (BMTB) specimens from 28 patients were revisited. Samples were evaluated for expression of CD3, CD20, CD138, CD68R, glycophorin C, CD42b, HHV8-latency-associated nuclear antigen (LANA1), Epstein–Barr virus-encoded small RNA and light chains. Presence of significant numbers of HHV8-LANA1+ lymphoid/plasmacytic cells, noted in 10/28 cases, was indicative of BM involvement and was associated with low CD4 and CD8 counts in peripheral blood. The characteristic morphological appearance of MCD seen in lymph nodes is a rare finding in BMTB. 4/5 cases with lymphoid aggregates were involved by MCD, whereas 6/23 cases without lymphoid aggregates were involved by MCD (P = 0·023). 9/18 cases with hypercellular marrow were involved by MCD, whilst only 1/8 cases with normo/hypocellular marrow showed involvement by MCD (P = 0·070). While 9/21 cases with increased marrow reticulin were involved by MCD, none of the cases with no increase in reticulin were involved by MCD (P = 0·080). Reactive plasmacytosis is a frequent finding. We conclude that bone marrow is involved in a significant proportion of patients with MCD (36%), and involvement can be identified by HHV8-LANA1 immunohistochemistry.
Issue Date: 8-Mar-2016
Date of Acceptance: 30-Oct-2015
URI: http://hdl.handle.net/10044/1/53298
DOI: https://dx.doi.org/10.1111/bjh.13919
ISSN: 1365-2141
Publisher: Wiley
Start Page: 923
End Page: 929
Journal / Book Title: British Journal of Haematology
Volume: 172
Issue: 6
Copyright Statement: This is the peer reviewed version of the following article: Ibrahim, H. A. H., Balachandran, K., Bower, M. and Naresh, K. N. (2016), Bone marrow manifestations in multicentric Castleman disease. Br J Haematol, 172: 923–929, which has been published in final form at https://dx.doi.org/10.1111/bjh.13919.  This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Sponsor/Funder: Imperial College Healthcare NHS Trust- BRC Funding
Funder's Grant Number: RDA02 79560
Keywords: Science & Technology
Life Sciences & Biomedicine
Hematology
bone marrow trephine biopsy
multicentric Castleman disease
human immunodeficiency virus
HHV8
Kaposi sarcoma
SARCOMA-ASSOCIATED HERPESVIRUS
KAPOSI-SARCOMA
HIV-INFECTION
POSITIVE PATIENTS
RITUXIMAB
INTERLEUKIN-6
CELLS
Adult
Aged
Antigens, Viral
Biopsy
Bone Marrow
Bone Marrow Examination
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Female
Giant Lymph Node Hyperplasia
HIV Infections
Herpesviridae Infections
Herpesvirus 8, Human
Humans
Immunophenotyping
Lymphocyte Count
Male
Middle Aged
Nuclear Proteins
Reticulin
1102 Cardiovascular Medicine And Haematology
Immunology
Publication Status: Published
Appears in Collections:Division of Cancer
Department of Medicine



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commonsx