The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species

Title: The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species
Author(s): Papanicolaou, A
Schetelig, MF
Arensburger, P
Atkinson, PW
Benoit, JB
Bourtzis, K
Castanera, P
Cavanaugh, JP
Chao, H
Childers, C
Curril, I
Huyen, D
Doddapaneni, H
Dolan, A
Dugan, S
Friedrich, M
Gasperi, G
Geib, S
Georgakilas, G
Gibbs, RA
Giers, SD
Gomulski, LM
Gonzalez-Guzman, M
Guillem-Amat, A
Han, Y
Hatzigeorgiou, AG
Hernandez-Crespo, P
Hughes, DST
Jones, JW
Karagkouni, D
Koskinioti, P
Lee, SL
Malacrida, AR
Manni, M
Mathiopoulos, K
Meccariello, A
Murali, SC
Murphy, TD
Muzny, DM
Oberhofer, G
Ortego, F
Paraskevopoulou, MD
Poelchau, M
Qu, J
Reczko, M
Robertson, HM
Rosendale, AJ
Rosselot, AE
Saccone, G
Salvemini, M
Savini, G
Schreiner, P
Scolari, F
Siciliano, P
Sim, SB
Tsiamis, G
Urena, E
Vlachos, IS
Werren, JH
Wimmer, EA
Worley, KC
Zacharopoulou, A
Richards, S
Handler, AM
Item Type: Journal Article
Abstract: Background The Mediterranean fruit fly (medfly), Ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. It exhibits a unique ability to invade and adapt to ecological niches throughout tropical and subtropical regions of the world, though medfly infestations have been prevented and controlled by the sterile insect technique (SIT) as part of integrated pest management programs (IPMs). The genetic analysis and manipulation of medfly has been subject to intensive study in an effort to improve SIT efficacy and other aspects of IPM control. Results The 479 Mb medfly genome is sequenced from adult flies from lines inbred for 20 generations. A high-quality assembly is achieved having a contig N50 of 45.7 kb and scaffold N50 of 4.06 Mb. In-depth curation of more than 1800 messenger RNAs shows specific gene expansions that can be related to invasiveness and host adaptation, including gene families for chemoreception, toxin and insecticide metabolism, cuticle proteins, opsins, and aquaporins. We identify genes relevant to IPM control, including those required to improve SIT. Conclusions The medfly genome sequence provides critical insights into the biology of one of the most serious and widespread agricultural pests. This knowledge should significantly advance the means of controlling the size and invasive potential of medfly populations. Its close relationship to Drosophila, and other insect species important to agriculture and human health, will further comparative functional and structural studies of insect genomes that should broaden our understanding of gene family evolution.
Publication Date: 22-Sep-2016
Date of Acceptance: 26-Aug-2016
URI: http://hdl.handle.net/10044/1/53082
DOI: https://dx.doi.org/10.1186/s13059-016-1049-2
ISSN: 1474-7596
Publisher: BioMed Central
Journal / Book Title: Genome Biology
Volume: 17
Copyright Statement: © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetics & Heredity
Medfly genome
Tephritid genomics
Insect orthology
Gene family evolution
Chromosomal synteny
Insect invasiveness
Insect adaptation
Medfly integrated pest management (IPM)
GERM-LINE TRANSFORMATION
BACTERIA-BINDING PROTEIN
SEMINAL FLUID PROTEINS
PROGRAMMED CELL-DEATH
DROSOPHILA-MELANOGASTER
ANOPHELES-GAMBIAE
TRANSPOSABLE ELEMENT
DIPTERA TEPHRITIDAE
MOLECULAR EVOLUTION
ANASTREPHA-SUSPENSA
Chromosomal synteny
Gene family evolution
Insect adaptation
Insect invasiveness
Insect orthology
Medfly genome
Medfly integrated pest management (IPM)
Tephritid genomics
Animals
Animals, Genetically Modified
Biological Evolution
Ceratitis capitata
Genome, Insect
High-Throughput Nucleotide Sequencing
Humans
Introduced Species
Molecular Sequence Annotation
Pest Control, Biological
Animals
Animals, Genetically Modified
Humans
Ceratitis capitata
Pest Control, Biological
Genome, Insect
Biological Evolution
Introduced Species
Molecular Sequence Annotation
High-Throughput Nucleotide Sequencing
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetics & Heredity
Medfly genome
Tephritid genomics
Insect orthology
Gene family evolution
Chromosomal synteny
Insect invasiveness
Insect adaptation
Medfly integrated pest management (IPM)
GERM-LINE TRANSFORMATION
BACTERIA-BINDING PROTEIN
SEMINAL FLUID PROTEINS
PROGRAMMED CELL-DEATH
DROSOPHILA-MELANOGASTER
ANOPHELES-GAMBIAE
TRANSPOSABLE ELEMENT
DIPTERA TEPHRITIDAE
MOLECULAR EVOLUTION
ANASTREPHA-SUSPENSA
05 Environmental Sciences
06 Biological Sciences
08 Information And Computing Sciences
Bioinformatics
Publication Status: Published
Article Number: 192
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