Large scale genomic analysis shows no evidence for pathogen adaptation between the blood and cerebrospinal fluid niches during bacterial meningitis.

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Title: Large scale genomic analysis shows no evidence for pathogen adaptation between the blood and cerebrospinal fluid niches during bacterial meningitis.
Author(s): Lees, JA
Kremer, PHC
Manso, AS
Croucher, NJ
Ferwerda, B
Serón, MV
Oggioni, MR
Parkhill, J
Brouwer, MC
Van der Ende, A
Van de Beek, D
Bentley, SD
Item Type: Journal Article
Abstract: Recent studies have provided evidence for rapid pathogen genome diversification, some of which could potentially affect the course of disease. We have previously described such variation seen between isolates infecting the blood and cerebrospinal fluid (CSF) of a single patient during a case of bacterial meningitis. Here, we performed whole-genome sequencing of paired isolates from the blood and CSF of 869 meningitis patients to determine whether such variation frequently occurs between these two niches in cases of bacterial meningitis. Using a combination of reference-free variant calling approaches, we show that no genetic adaptation occurs in either invaded niche during bacterial meningitis for two major pathogen species, Streptococcus pneumoniae and Neisseria meningitidis. This study therefore shows that the bacteria capable of causing meningitis are already able to do this upon entering the blood, and no further sequence change is necessary to cross the blood-brain barrier. Our findings place the focus back on bacterial evolution between nasopharyngeal carriage and invasion, or diversity of the host, as likely mechanisms for determining invasiveness.
Publication Date: 31-Jan-2017
Date of Acceptance: 18-Dec-2016
URI: http://hdl.handle.net/10044/1/52705
DOI: https://dx.doi.org/10.1099/mgen.0.000103
ISSN: 2057-5858
Publisher: Microbiology Society
Journal / Book Title: Microbial Genomics
Volume: 3
Issue: 1
Copyright Statement: ã 2017 The Authors This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 104169/Z/14/Z
Keywords: Neisseria meningitidis
Streptococcus pneumoniae
ivr locus
meningitis
pan-genome calling
within-host evolution
Neisseria meningitidis
Streptococcus pneumoniae
ivr locus
meningitis
pan-genome calling
within-host evolution
Publication Status: Published online
Article Number: e000103
Appears in Collections:Epidemiology, Public Health and Primary Care



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