Altmetric

Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data-independent acquisition strategy

File Description SizeFormat 
Lee final accepted draft.pdfAccepted version2.51 MBAdobe PDFView/Open
Title: Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data-independent acquisition strategy
Authors: Gethings, LA
Richardson, K
Wildgoose, J
Lennon, S
Jarvis, S
Bevan, CL
Vissers, JPC
Langridge, JI
Item Type: Journal Article
Abstract: Rationale A novel data-independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time-of-flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches. Methods Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented. Results The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data. Conclusions In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification.
Issue Date: 4-Sep-2017
Date of Acceptance: 10-Jul-2017
URI: http://hdl.handle.net/10044/1/52532
DOI: https://dx.doi.org/10.1002/rcm.7941
ISSN: 0951-4198
Publisher: Wiley
Start Page: 1599
End Page: 1606
Journal / Book Title: Rapid Communications in Mass Spectrometry
Volume: 31
Issue: 19
Copyright Statement: This is the peer reviewed version of the following article: Gethings LA, Richardson K, Wildgoose J, et al. Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data-independent acquisition strategy. Rapid Commun Mass Spectrom. 2017;31:1599-1606. , which has been published in final form at https://dx.doi.org/10.1002/rcm.7941.  This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Sponsor/Funder: Wellcome Trust
Genesis Research Trust
Funder's Grant Number: 102758/Z/13/Z
01070
Keywords: 03 Chemical Sciences
04 Earth Sciences
06 Biological Sciences
Analytical Chemistry
Publication Status: Published
Appears in Collections:Division of Surgery
Division of Cancer
Faculty of Medicine



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commonsx