Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis

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Title: Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis
Authors: Molyneaux, PL
Cox, MJ
Wells, AU
Kim, HC
Ji, W
Cookson, WOC
Moffatt, MF
Kim, DS
Maher, TM
Item Type: Journal Article
Abstract: Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF.
Issue Date: 1-Feb-2017
Date of Acceptance: 16-Jan-2017
ISSN: 1465-9921
Publisher: BioMed Central
Journal / Book Title: Respiratory Research
Volume: 18
Copyright Statement: © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.
Sponsor/Funder: Medical Research Council (MRC)
Royal Brompton & Harefield NHS Foundation Trust
Medical Research Council (MRC)
National Institute for Health Research
National Institute for Health Research
Funder's Grant Number: G1000758
Keywords: Science & Technology
Life Sciences & Biomedicine
Respiratory System
Idiopathic pulmonary fibrosis
Acute exacerbation
Bacterial infection
1102 Cardiovascular Medicine And Haematology
1103 Clinical Sciences
Publication Status: Published
Open Access location:
Article Number: 29
Appears in Collections:National Heart and Lung Institute

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