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Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis

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Title: Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis
Authors: Molyneaux, PL
Cox, MJ
Wells, AU
Kim, HC
Ji, W
Cookson, WOC
Moffatt, MF
Kim, DS
Maher, TM
Item Type: Journal Article
Abstract: Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF.
Issue Date: 1-Feb-2017
Date of Acceptance: 16-Jan-2017
URI: http://hdl.handle.net/10044/1/52241
DOI: https://dx.doi.org/10.1186/s12931-017-0511-3
ISSN: 1465-9921
Publisher: BioMed Central
Journal / Book Title: Respiratory Research
Volume: 18
Replaces: http://hdl.handle.net/10044/1/52223
10044/1/52223
Copyright Statement: © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Sponsor/Funder: Medical Research Council (MRC)
Royal Brompton & Harefield NHS Foundation Trust
Medical Research Council (MRC)
National Institute for Health Research
National Institute for Health Research
Funder's Grant Number: G1000758
B0479
G1000758
NF-SI-0512-10126
n/a
Keywords: Science & Technology
Life Sciences & Biomedicine
Respiratory System
Idiopathic pulmonary fibrosis
Acute exacerbation
Bacterial infection
16S
DISEASE
1102 Cardiovascular Medicine And Haematology
1103 Clinical Sciences
Publication Status: Published
Open Access location: https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-017-0511-3
Article Number: 29
Appears in Collections:National Heart and Lung Institute



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