Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models

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Title: Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models
Authors: Allocca, G
Kusumbe, AP
Ramasamy, SK
Wang, N
Item Type: Journal Article
Abstract: Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.
Issue Date: 7-Dec-2016
Date of Acceptance: 2-Nov-2016
URI: http://hdl.handle.net/10044/1/52075
DOI: https://dx.doi.org/10.1038/bonekey.2016.84
ISSN: 2047-6396
Publisher: Nature Publishing Group
Journal / Book Title: BoneKEy Reports
Volume: 5
Copyright Statement: © 2016 International Bone & Mineral Society All rights reserved
Keywords: Science & Technology
Life Sciences & Biomedicine
Orthopedics
IN-VIVO MODELS
HEMATOPOIETIC STEM
PROSTATE-CANCER
3-DIMENSIONAL RECONSTRUCTION
2-PHOTON EXCITATION
PROGENITOR CELLS
METASTASIS
NICHE
ANGIOGENESIS
OSTEOGENESIS
Publication Status: Published
Article Number: 851
Appears in Collections:Clinical Sciences
Molecular Sciences



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